Construct: ORF TRCN0000465871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015980.1_s317c1
- Derived from:
- ccsbBroadEn_10239
- DNA Barcode:
- TTAGCTCTTCTGCGATGGGTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAND2P (54581)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54581 | SCAND2P | SCAN domain containing 2 ps... | NR_003654.2 | 11.5% | 1_440del;897_3951del | |
| 2 | human | 54581 | SCAND2P | SCAN domain containing 2 ps... | NR_004859.1 | 9.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtagctgtg gaccaacaaa tccagactcc ttcagtacaa gatctccaaa 121 tagttaaact ggaagaagat tcccactggg agcaggaaat ttcccttcaa gggaattacc 181 ctggaccaga gacatcctgc cagagctttt ggcatttccg ttaccaagaa gcatcacgac 241 cccgagaggc ccTCCTCCAG CTCCAGAAGC TCTGTTGTCA GTGGCTAAGG CCAGAGAAGT 301 GTACAAAAGA GCAGATCCTG GAGTTGCTGG TCCTAGAACA GTTCCCGACT GTCCTTCTCC 361 AGGAGATCCA GATCTGGGTC AGACAGCAGC ATCCGGAGAG TGGAGAGGAG GCAGTGGCCC 421 TGGTGGAAGA CTTGCAGAAA GAACCTGGAA GACAGAGGCT GGAGCCCTGC CTGATGTGGC 481 TCTGGGAATT CCTGCAGAGA AGAGCAGGGG TGGCCAGGAG ATACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGATTA GCTCTTCTGC GATGGGTGTT TACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t