Construct: ORF TRCN0000465877
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016340.1_s317c1
- Derived from:
- ccsbBroadEn_08615
- DNA Barcode:
- AACAGAGGGTGCTGCATACATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG12 (55970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465877
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55970 | GNG12 | G protein subunit gamma 12 | NM_018841.6 | 99.5% | 98.6% | 85A>G |
| 2 | human | 55970 | GNG12 | G protein subunit gamma 12 | XM_017001809.2 | 99.5% | 98.6% | 85A>G |
| 3 | human | 55970 | GNG12 | G protein subunit gamma 12 | XM_024448405.1 | 99.5% | 98.6% | 85A>G |
| 4 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177556.1 | 86.1% | 95.8% | (many diffs) |
| 5 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177557.1 | 86.1% | 95.8% | (many diffs) |
| 6 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177558.1 | 86.1% | 95.8% | (many diffs) |
| 7 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177559.1 | 86.1% | 95.8% | (many diffs) |
| 8 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177560.1 | 86.1% | 95.8% | (many diffs) |
| 9 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_025278.5 | 86.1% | 95.8% | (many diffs) |
| 10 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | XM_006505542.3 | 86.1% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 179
- ORF end:
- 395
- ORF length:
- 216
- Sequence:
-
1 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 61 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 121 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 181 GTCCAGCAAA ACAGCAAGCA CCAACAATAT AGCCCAGGCA AGGAGAACTG TGCAGCAGTT 241 AAGATTAGAA GCCTCCATTG AAGGAATAAA GGTTTCGAAG GCATCAGCGG ACCTCATGTC 301 CTACTGTGAG GAACATGCCA GGAGTGACCC TTTGCTGATA GGAATACCAA CTTCAGAAAA 361 CCCTTTCAAG GATAAAAAAA CTTGCATCAT CTTATACCCA ACTTTCTTGT ACAAAGTGGT 421 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 481 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 541 AACAGAGGGT GCTGCATACA TATCACGCGT TAAGTCgaca atcaacctct ggattacaaa 601 atttgtgaaa gatt