Construct: ORF TRCN0000465881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004773.1_s317c1
- Derived from:
- ccsbBroadEn_05599
- DNA Barcode:
- TGAGTTATTATACCGCAGATCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MUSTN1 (389125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389125 | MUSTN1 | musculoskeletal, embryonic ... | NM_205853.4 | 99.5% | 98.7% | 60C>G |
| 2 | human | 100526772 | STIMATE-MUSTN1 | STIMATE-MUSTN1 readthrough | NM_001198974.3 | 21.4% | 20.9% | (many diffs) |
| 3 | mouse | 66175 | Mustn1 | musculoskeletal, embryonic ... | NM_181390.3 | 85.3% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 339
- ORF length:
- 246
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGTCCCA GGCTGGTGCT CAGGAAGCCC 121 CTATCAAGAA GAAGCGCCCC CCTGTGAAGG AGGAGGACCT GAAGGGGGCC CGAGGAAACC 181 TGACCAAGAA CCAGGAAATC AAGTCCAAGA CCTACCAGGT CATGCGAGAG TGTGAGCAAG 241 CTGGCTCGGC CGCCCCGTCG GTGTTCAGCC GCACCCGCAC AGGTACCGAG ACTGTCTTTG 301 AGAAGCCCAA AGCCGGACCC ACCAAGAGTG TCTTCGGCTT GCCAACTTTC TTGTACAAAG 361 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 421 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 481 ACGATGAGTT ATTATACCGC AGATCAAGAC GCGTTAAGTC gacaatcaac ctctggatta 541 caaaatttgt gaaagatt