Construct: ORF TRCN0000465892

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006005.1_s317c1
Derived from:
ccsbBroadEn_11950
DNA Barcode:
TGACTCGGTCACCGGAGAGGATTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHF20L1 (51105)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465892
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_017013513.1 36.1% 36.1% 1_1707del;2371_2373delAAG
2 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_017013514.2 36.1% 36.1% 1_1707del;2371_2373delAAG
3 human 51105 PHF20L1 PHD finger protein 20 like 1 NM_001277196.2 32.5% 32.5% 1_2004del
4 human 51105 PHF20L1 PHD finger protein 20 like 1 NM_001362971.1 32.5% 32.5% 1_2004del
5 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517081.1 32.5% 32.5% 1_2004del;2668_2670delAAG
6 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_017013511.1 32.5% 32.5% 1_2004del;2668_2670delAAG
7 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517080.1 32.5% 32.5% 1_2007del;2671_2673delAAG
8 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_017013510.1 32.5% 32.5% 1_2007del;2671_2673delAAG
9 human 51105 PHF20L1 PHD finger protein 20 like 1 NM_016018.5 31.7% 31.7% 1_2082del
10 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517079.1 31.7% 31.7% 1_2082del;2746_2748delAAG
11 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_017013509.1 31.7% 31.7% 1_2082del;2746_2748delAAG
12 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517078.1 31.6% 31.6% 1_2085del;2749_2751delAAG
13 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_005250931.1 31.6% 31.6% 1_2097del
14 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517077.1 31.6% 31.6% 1_2094del;2758_2760delAAG
15 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517075.1 31.5% 31.5% 1_2097del;2761_2763delAAG
16 human 51105 PHF20L1 PHD finger protein 20 like 1 XM_011517076.1 31.5% 31.5% 1_2097del;2761_2763delAAG
17 human 51105 PHF20L1 PHD finger protein 20 like 1 XR_001745536.1 15.2% 1_2500del;3470_6356del
18 human 51105 PHF20L1 PHD finger protein 20 like 1 XR_001745535.1 15.2% 1_2515del;3485_6371del
19 human 51105 PHF20L1 PHD finger protein 20 like 1 NR_156424.2 12.5% 1_2298del;2407_2408ins196;3072_5958del
20 human 51105 PHF20L1 PHD finger protein 20 like 1 XR_001745533.1 12.3% 1_2392del;2501_2502ins196;3166_6052del
21 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520921.3 27.8% 27.6% (many diffs)
22 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_017316601.1 27.8% 27.6% (many diffs)
23 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520920.3 27.8% 27.5% (many diffs)
24 mouse 239510 Phf20l1 PHD finger protein 20-like 1 NM_001081409.1 27.1% 26.9% (many diffs)
25 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520919.3 27% 26.8% (many diffs)
26 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520918.3 27% 26.8% (many diffs)
27 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520916.3 26.9% 26.7% (many diffs)
28 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XM_006520917.3 26.9% 26.7% (many diffs)
29 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781524.1 9.6% (many diffs)
30 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781525.1 9.5% (many diffs)
31 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781528.1 9.5% (many diffs)
32 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781531.1 9.4% (many diffs)
33 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781529.1 9.3% (many diffs)
34 mouse 239510 Phf20l1 PHD finger protein 20-like 1 XR_001781530.1 9.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1035
ORF length:
969
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat ccagtgtgaa gagtgcttgt gttggcaaca cagcgtgtgc atggggctgc
121tggaggagag cattccagag cagtacatct gctatatctg ccgggaccca ccaggtcaga
181ggtggagtgc aaaatatcgt tatgataagg agtggttgaa taatgggaga atgtgcgggt
241tatcattttt caaagaaaat tattctcatc tcaatgccaa aaagatagtt tctacacatc
301acctgcttgc tgatgtctat ggtgttacag aagtgctaca cgggctacag ctgaagattg
361gaatactaaa gaataaacat catcctgacc ttcatctctg ggcttgttcc gggaagcgaa
421aagaccaaga tcaaataata gctggggtgg agaaaaaaat agctcaagac acagttaatc
481gagaagaaaa gaaatatgta cagaaccata aagaaccacc tcgtttgccc ctaaaaatgg
541aaggaactta tataacaagt gagcatagct atcaaaagcc acaaagtttt ggtcaggact
601gtaaatctct cgcagaccct gggagctcag atgatgatga tgttagtagt ttggaagaag
661aacaagaaTT CCACATGAGA AGTAAAAACA GTTTACAGTA CTCAGCAAAA GAACATGGAA
721TGCCTGAAAA GAATCCAGCT GAAGGGAATA CAGTATTTGT TTATAATGAT AAAAAGGGCA
781CCGAAGACCC AGGAGACTCA CATCTTCAGT GGCAGCTCAA TCTCCTTACA CACATAGAAA
841ATGTGCAGAA CGAAGTTACC AGCAGGATGG ACCTAATAGA AAAAGAAGTC GATGTTCTGG
901AAAGCTGGCT TGATTTCACA GGGGAGTTGG AGCCACCAGA TCCTCTTGCA AGATTGCCCC
961AACTTAAACG CCACATAAAA CAGCTCCTAA TTGACATGGG CAAAGTACAG CAGATAGCAA
1021CTCTTTGCTC TGTATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1081CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1141TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGACTCGGTC ACCGGAGAGG
1201ATTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.