Construct: ORF TRCN0000465902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004362.1_s317c1
- Derived from:
- ccsbBroadEn_01238
- DNA Barcode:
- ACATAGTAGAATTAAACCTCGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2D (5433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5433 | POLR2D | RNA polymerase II subunit D | NM_004805.4 | 100% | 100% | |
| 2 | mouse | 69241 | Polr2d | polymerase (RNA) II (DNA di... | NM_027002.3 | 87.5% | 100% | (many diffs) |
| 3 | mouse | 69241 | Polr2d | polymerase (RNA) II (DNA di... | XM_017317977.1 | 67.3% | 77.4% | (many diffs) |
| 4 | mouse | 69241 | Polr2d | polymerase (RNA) II (DNA di... | NM_027101.2 | 64.7% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgggtggc agcgatccgc gggctggcga cgtagaggag gacgccTCAC 121 AGCTCATCTT TCCTAAAGAG TTTGAAACAG CTGAGACACT TCTAAATTCA GAAGTTCATA 181 TGCTTCTGGA ACATCGAAAG CAGCAGAATG AGAGTGCAGA GGACGAACAG GAGCTCTCAG 241 AAGTCTTCAT GAAAACATTA AACTACACAG CCCGTTTCAG TCGTTTCAAA AACAGAGAGA 301 CCATTGCCAG TGTTCGTAGC TTGCTACTCC AGAAAAAGCT TCATAAGTTT GAGTTGGCCT 361 GTTTGGCCAA CCTTTGCCCA GAGACTGCTG AGGAGTCCAA GGCTCTAATC CCAAGCTTGG 421 AGGGACGGTT TGAAGATGAG GAGCTGCAGC AGATTCTTGA TGATATCCAG ACAAAGCGCA 481 GCTTTCAGTA TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA TAGTAGAATT AAACCTCGTA 661 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t