Construct: ORF TRCN0000465913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008145.3_s317c1
- Derived from:
- ccsbBroadEn_14711
- DNA Barcode:
- ACTGATCCGGAGACCTCTTGCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEK3 (4752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_002498.3 | 100% | 100% | |
| 2 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_152720.2 | 100% | 100% | |
| 3 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_001146099.1 | 96.6% | 96.6% | 876_877ins51 |
| 4 | human | 4752 | NEK3 | NIMA related kinase 3 | NR_164641.1 | 68.9% | 1_112del;989_990ins50;1581_2078del | |
| 5 | human | 4752 | NEK3 | NIMA related kinase 3 | NR_027415.2 | 62.8% | 1_376del;1245_1246insA;1894_2412del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1584
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgactacatg gtcctgagaa tgattgggga gggctccttc ggcagagctc 121 ttttggttca gcatgaaagc agtaatcaga tgtttgccat gaaagaaata aggcttccca 181 agtctttctc taatacacag aattctagga aggaggctgt tcttttagcc aaaatgaaac 241 accctaatat tgttgccttc aaagaatcat ttgaagctga aggacacttg tatattgtga 301 tggaatactg tgatggaggg gatctaatgc aaaagattaa acagcagaaa ggaaagttat 361 ttcctgaaga catgatactt aattggttta cccaaatgtg ccttggagta aatcacattc 421 acaagaaacg tgtgctacac agagatatca agtccaagaa tatcttcctc actcagaatg 481 gaaaagtgaa attgggagac tttggatctg cccgtcttct ctccaatccg atggcatttg 541 cttgtaccta tgtgggaact ccttattatg tgcctccaga aatttgggaa aacctgcctt 601 ataacaataa aagtgacatc tggtccttgg gttgcatcct gtatgaactc tgtaccctta 661 agcatccatt tcaggcaaat agttggaaaa atcttatcct caaagtatgt caagggtgca 721 tcagtccact gccgtctcat tactcctatg aacttcagtt cctagtcaag cagatgttta 781 aaaggaatcc ctcacatcgc ccctcggcta caacgcttct ctctcgaggc atcgtagctc 841 ggcttgtcca gaagtgctta ccccccgaga tcatcatgga atatggtgag gaagtattag 901 aagaaataaa aaattcgaag cataacacac caagaaaaaa aacaaacccc agcagaatca 961 ggatagcttt gggaaatgaa gcaagcacag tgcaagagga agaacaagat agaaagggta 1021 gccatactga tttggaaagc attaatgaaa atttagttga aagtgcattg agaagagtaa 1081 acagagaaga aaaaggtaat aagtcagtcc atctgaggaa agccagttca ccaaatcttc 1141 atagacgaca gtgggagaaa aatgtaccca atacagctct tacagctttg gaaaatgcat 1201 ccatactcac ctccagttta acagcagagg acgatagagg tggttctgta ataaagtaca 1261 gcaaaaatac tactcgtaag cagtggctca aagagacccc tgacactttg ttgaacatcc 1321 ttaagaatgc tgatcTCAGC TTGGCTTTTC AAACATACAC AATATATAGA CCAGGTTCAG 1381 AAGGGTTCTT GAAAGGCCCC CTGTCTGAAG AAACAGAAGC ATCGGACAGT GTTGATGGAG 1441 GTCACGATTC TGTCATTTTG GATCCAGAGC GACTTGAGCC TGGGCTAGAT GAGGAGGACA 1501 CGGACTTTGA GGAGGAAGAT GACAACCCCG ACTGGGTGTC AGAGCTGAAG AAGCGAGCTG 1561 GATGGCAAGG CCTGTGCGAC AGATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTGATCCGGA 1741 GACCTCTTGC GGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att