Construct: ORF TRCN0000465926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002717.1_s317c1
- Derived from:
- ccsbBroadEn_07413
- DNA Barcode:
- TTATATACAGTCTTATCATTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEX16 (9409)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9409 | PEX16 | peroxisomal biogenesis fact... | NM_004813.3 | 99.9% | 100% | 873T>C |
| 2 | human | 9409 | PEX16 | peroxisomal biogenesis fact... | NM_057174.2 | 92.8% | 92.2% | (many diffs) |
| 3 | mouse | 18633 | Pex16 | peroxisomal biogenesis fact... | NM_145122.2 | 86.9% | 91% | (many diffs) |
| 4 | mouse | 18633 | Pex16 | peroxisomal biogenesis fact... | XM_017316343.1 | 53.4% | 55% | (many diffs) |
| 5 | mouse | 18633 | Pex16 | peroxisomal biogenesis fact... | XR_001781473.1 | 52.2% | (many diffs) | |
| 6 | mouse | 18633 | Pex16 | peroxisomal biogenesis fact... | XM_006498925.2 | 48.7% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagctgcgg ctcctgggcc tccgctacca ggagtacgtg actcgtcacc 121 cggccgccac ggcccagctg gagacagcag tgcggggctt cagttacctg ctggcaggtc 181 gattcgccga ttcgcacgag ctgtcagagc tggtgtactc tgcctctaac ctgcttgtgc 241 tgctcaatga cgggatccta cggaaggagc ttcggaaaaa gttgcctgtg tcgctgtccc 301 agcagaagct gctgacatgg ctgagcgtgc tggagtgcgt ggaggtgttc atggagatgg 361 gagctgccaa ggtgtggggt gaagtgggcc gctggcttgt catcgccctc atccagctgg 421 ccaaggctgt actgcggatg ctcctgctgc tctggttcaa ggctggcctc cagacttcac 481 cccctatcgt tccactggac agagagaccc aggcacagcc cccggatggt gaccacagcc 541 ctggcaacca tgagcagtcc tacgtgggga agcggtcaaa ccgggtggtg cgaaccctcc 601 agaacacgcc gtccctgcac tccaggcact ggggagctcc ccagcagcgg gagggacggc 661 agcagcagca tcacgaggag ctgagtgcga cccccacccc cctggggctg caggagacca 721 tcgcagagtt tttgtacatt gcccggccgc tgctgcactt gctcagcctg ggcctgtggg 781 gtcagaggtc gtggaaaccc tggctcttgg ctggtgttgt ggacgtgacc agcctgagcc 841 TCCTGAGTGA CAGAAAGGGC CTGACCCGGA GGGAGCGGCG GGAGCTGCGG CGCCGGACCA 901 TCCTGCTGCT CTACTACCTG CTGCGCTCTC CTTTCTACGA CCGCTTCTCC GAGGCCAGGA 961 TCCTCTTCCT GCTCCAGTTG CTGGCCGACC ACGTCCCTGG CGTTGGCCTG GTCACAAGGC 1021 CGCTCATGGA TTACTTGCCC ACCTGGCAGA AAATCTACTT CTACAGTTGG GGCTGCCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGTTTCTT GGCTTTATAT 1201 ATCTTGTGGA AAGGACGATT ATATACAGTC TTATCATTAA CCACGCGTTA AGTCgacaat 1261 caacctctgg attacaaaat ttgtgaaaga tt