Construct: ORF TRCN0000465929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012058.1_s317c1
- Derived from:
- ccsbBroadEn_12401
- DNA Barcode:
- CAAGTCACTTTGCCATTGTGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCOA5 (57727)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57727 | NCOA5 | nuclear receptor coactivator 5 | NM_001348151.2 | 95% | 93.6% | (many diffs) |
| 2 | human | 57727 | NCOA5 | nuclear receptor coactivator 5 | NM_001348150.2 | 91.8% | 91.8% | 1_84del |
| 3 | human | 57727 | NCOA5 | nuclear receptor coactivator 5 | NM_001348149.2 | 77.5% | 70.4% | 1_130del;190_332del |
| 4 | human | 57727 | NCOA5 | nuclear receptor coactivator 5 | NM_001348148.2 | 58.8% | 55.7% | (many diffs) |
| 5 | human | 57727 | NCOA5 | nuclear receptor coactivator 5 | NM_020967.3 | 52.3% | 46.1% | (many diffs) |
| 6 | mouse | 228869 | Ncoa5 | nuclear receptor coactivator 5 | XM_006499369.3 | 69.4% | 69.9% | (many diffs) |
| 7 | mouse | 228869 | Ncoa5 | nuclear receptor coactivator 5 | NM_144892.1 | 46.7% | 45.8% | (many diffs) |
| 8 | mouse | 228869 | Ncoa5 | nuclear receptor coactivator 5 | XM_017317897.1 | 44.7% | 42.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggatgccc gggacagcag agacattcga gacccccgag acttgcggga 121 ccacagacat gagagactcc cgagatccta tgtacaggag agaaggctct tatgaccgat 181 acctacgaat ggatgactat tgcaggagaa aggatgactc ttattttgac cgttacagag 241 atagctttga tggacggggc cctccaggcc cagaaagtca gtctcgtgca aaagagcgtt 301 tgaaacgtga ggaacggcgt agagaagagc tttatcgtca atattttgag gaaatccaga 361 gacgctttga tgccgaaagg cccgttgatt gttctgtgat tgtggtcaac aaacagacaa 421 aagactatgc tgagtctgtg gggcggaagg tgcgagacct gggcatggta gtggacttga 481 tcttccttaa cacagaagtg tcactgtcac aagccttgga ggatgttagc aggggaggtt 541 ctccttttgc tattgtcatc acccagcaac accagattca ccgctcctgc acagtcaaca 601 tcatgtttgg aaccccgcaa gagcatcgca acatgcccca agcagatgcc atggtgctgg 661 tggCCAGAAA TTATGAGCGT TACAAGAATG AGTGCCGGGA GAAGGAACGT GAGGAGATTG 721 CCAGACAGGC AGCCAAGATG GCCGATGAAG CCATCCTGCA GGAAAGAGAG AGAGGAGGCC 781 CTGAGGAGGG AGTGCGTGGG GGCCACCCTC CAGCCATCCA GAGCCTCATC AACCTGCTGG 841 CAGACAACAG GTACCTCACT GCTGAAGAGA CTGACAAGAT CATCAACTAC CTGCGAGAGC 901 GGAAGGAGCG GCTGATGAGG AGCAGCACCG ACTCTCTGCC TGGTGAGCTA CGTGGCAGGG 961 CCGAGGCCCG ATTTCCCGCC AACCACTCGG GGCGACCTCG GGTGCCTCGC TGCCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGACAAG TCACTTTGCC ATTGTGTCTT ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt