Construct: ORF TRCN0000465934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001266.1_s317c1
- Derived from:
- ccsbBroadEn_08540
- DNA Barcode:
- AGGGATCCAAATCCGCCCCGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELAC1 (55520)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55520 | ELAC1 | elaC ribonuclease Z 1 | NM_018696.3 | 99.9% | 99.7% | 16A>T |
| 2 | mouse | 114615 | Elac1 | elaC ribonuclease Z 1 | NM_053255.3 | 86.4% | 91.4% | (many diffs) |
| 3 | mouse | 114615 | Elac1 | elaC ribonuclease Z 1 | XR_385970.3 | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1156
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 gttggcatgt ctatggatgt gtcattcctg gggacgggtg cagcataccc atctccaacc 121 cggggtgcct ctgctgtggt ccttcggtgt gaaggcgagt gctggctctt tgactgtggg 181 gagggaacac agacacagct tatgaaaagc caacttaaag cagggagaat taccaagatc 241 ttcatcacac accttcatgg agaccatttc tttggccttc ctgggctcct ctgcacaatc 301 agcctgcaga gtggctccat ggtgtccaaa cagcctattg aaatctatgg ccctgtaggg 361 cttcgggact ttatctggcg aaccatggaa ctctctcaca cggagctggt cttccattat 421 gtggttcatg aactggttcc tacagcagat caatgtcctg cagaagaact aaaagaattt 481 gcgcatgtga atagagcaga cagtcctccc aaagaggaac aaggaagaac tatcctgtta 541 gactcagaag aaaactcata ccttctgttt gatgatgaac aatttgttgt aaaagcattt 601 cgcctctttc acagaattcc ctcatttggg ttttcagtcg tggaaaagaa acgcccaggt 661 aaactcaatg cacagaaact taaagacctt ggtgttccac caggtcctgc ctatgggaag 721 ctgaaaaatg gaattTCTGT TGTTCTGGAA AATGGGGTTA CAATTTCTCC CCAAGATGTC 781 TTAAAAAAGC CTATTGTTGG AAGAAAAATC TGCATATTGG GTGACTGCTC TGGGGTTGTG 841 GGTGATGGAG GAGTAAAACT GTGCTTTGAA GCAGACCTGT TGATCCACGA AGCAACCCTG 901 GATGATGCCC AGATGGACAA AGCAAAGGAG CATGGCCACA GCACACCACA GATGGCAGCA 961 ACATTTGCAA AGTTGTGCCG TGCAAAGAGG CTGGTTCTGA CTCACTTCAG TCAGAGGTAC 1021 AAACCAGTTG CCTTGGCCAG AGAAGGAGAA ACAGATGGCA TTGCAGAACT AAAAAAGCAA 1081 GCTGAATCAG TGTTAGATCT CCAAGAAGTG ACTCTAGCAG AAGATTTTAT GGTGATAAGC 1141 ATTCCAATCA AGAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 1201 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 1261 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGGGATCCA AATCCGCCCC 1321 GTTACACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt