Construct: ORF TRCN0000465939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011696.1_s317c1
- Derived from:
- ccsbBroadEn_02320
- DNA Barcode:
- CCTATTAGCTTAATGCGATACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTR1A (10121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465939
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10121 | ACTR1A | actin related protein 1A | NM_005736.4 | 100% | 100% | |
| 2 | human | 10120 | ACTR1B | actin related protein 1B | NM_005735.4 | 79.6% | 90.4% | (many diffs) |
| 3 | mouse | 54130 | Actr1a | ARP1 actin-related protein ... | NM_016860.1 | 91.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtcctacgat gtgatcgcca accagcctgt cgtgatcgac aacggatccg 121 gtgtgattaa agctggtttt gctggtgatc agatccccaa atactgcttt ccaaactatg 181 tgggccgacc caagcacgtt cgtgtcatgg caggagccct tgaaggcgac atcttcattg 241 gccccaaagc tgaggagcac cgagggctgc tttcaatccg ctatcccatg gagcatggca 301 tcgtcaagga ttggaacgac atggaacgca tttggcaata tgtctattct aaggaccagc 361 tgcagacttt ctcagaggag catcctgtgc tcctgactga ggcgccttta aacccacgaa 421 aaaaccggga acgagctgcc gaagttttct tcgagacctt caatgtgccc gctcttttca 481 tctccatgca agctgtactc agcctttacg ctacaggcag gaccacaggg gtggtgctgg 541 attctgggga tggagtcacc catgctgtgc ccatctatga gggctttgcc atgccccact 601 ccatcatgcg catcgacatc gcgggccggg acgtctctcg cttcctgcgc ctctacctgc 661 gtaaggaggg ctacgacttc cactcatcct ctgagtttga gattgtcaag gccataaaag 721 aaagagcctg ttacctatcc ataaaccccc aaaaggatga gacgctagag acagagaaag 781 ctcagtacta cctgcctgat ggcagcacca ttgagattgg TCCTTCCCGA TTCCGGGCCC 841 CTGAGTTGCT CTTCAGGCCA GATTTGATTG GAGAGGAGAG TGAAGGCATC CACGAGGTCC 901 TGGTGTTCGC CATTCAGAAG TCAGACATGG ACCTGCGGCG CACGCTTTTC TCTAACATTG 961 TCCTCTCAGG AGGCTCTACC CTGTTCAAAG GTTTTGGTGA CAGGCTCCTG AGTGAAGTGA 1021 AGAAACTAGC TCCAAAAGAT GTGAAGATCA GGATATCTGC ACCTCAGGAG AGACTGTATT 1081 CCACGTGGAT TGGGGGCTCC ATCCTTGCCT CCCTGGACAC CTTTAAGAAG ATGTGGGTCT 1141 CCAAAAAGGA ATATGAGGAA GACGGTGCCC GATCCATCCA CAGAAAAACC TTCTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAC CTATTAGCTT AATGCGATAC TACACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att