Construct: ORF TRCN0000465948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018927.2_s317c1
- Derived from:
- ccsbBroadEn_14634
- DNA Barcode:
- GCCACACCTCTAGATATCTCACAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ERN1 (2081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | NM_001433.5 | 99.1% | 25.2% | (many diffs) |
| 2 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024347.2 | 95% | 22.9% | (many diffs) |
| 3 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024348.2 | 91.7% | 19.6% | (many diffs) |
| 4 | mouse | 78943 | Ern1 | endoplasmic reticulum (ER) ... | NM_023913.2 | 87.9% | 24.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 825
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggcccgg cggctgctgc tgctgctgac gctgctgctg cccggcctcg 121 ggatttttgg aagtaccagc acagtgacgc ttcctgaaac cttgttgttt gtgtcaacgc 181 tggatggaag tttgcatgct gtcagcaaga ggacaggctc aatcaaatgg actttaaaag 241 aagatccagt cctgcaggtc ccaacacatg tggaagagcc tgcctttctc ccagatccta 301 atgatggcag cctgtatacg cttggaagca agaataatga aggcctgacg aaacttcctt 361 ttaccatccc agaattggtg caggcatccc catgccgaag ttcagatgga atcctctaca 421 tgggtaaaaa gcaggacatc tggtatgtta ttgacctcct gaccggagag aagcagcaga 481 ctttgtcatc ggcctttgca gatagtctct gcccatcaac ctctcttctg tatcttgggc 541 gaacagaata caccatcacc atgtacgaca ccaaaacccg agagctccgg tggaatgcca 601 cctactttga ctatgcggcc tcactgcctg aggacgacgt ggactacaag atgtcccact 661 ttgtgtccaa tggtgatggg ctggtggtga ctgtggacag tgaatctggg gacgtcctgt 721 ggatccaaaa ctacgcctcc cctgtggtgg ccttttatgt ctggcagcgg gagggtctga 781 ggaaggtgat gcacatcaat gtcgctgtga gaccctgcgc tatctgacct tcatgtctgg 841 gggaggtggg ggcgcatcac aaagtggaag tacccgttcc ccaaagagac agaggccaag 901 agcaagctga cgcccactct gtatgttggg aaatactcta ccagcctcta tgcctctccc 961 tcaatggtac acgagggggt tgctgtcgtg ccccgcggca gcacacttcc tttgctggaa 1021 gggccccaga ctgatggcgt caccatcggg gacaaggggg agtgtgtgat cacgcccagc 1081 acggacgtca agtttgatcc cggactcaaa agcaagaaca agctcaacta cttgaggaat 1141 tactggcttc tgataggaca ccatgaaacc ccactgtctg cgtctaccaa gatgctggag 1201 agatttccca acaatctacc caaacatcgg gaaaatgtga ttcctgctga ttcagagaaa 1261 aagagctttg aggaagttat caacctggtt gaccagactt cagaaaacgc acctaccacc 1321 gtgtctcggg atgtggagga gaagcccgcc catgcccctg cccggcccga ggcccccgtg 1381 gactccatgc ttaaggacat ggctaccatc atcctgagca ccttcctgct gattggctgg 1441 gtggccttca tcatcaccta tcccctgagc atgcatcagc agcagcagct ccagcaccag 1501 cagttccaga aggaactgga gaagatccag ctcctgcagc agcagcagca gcagctgccc 1561 ttccacccac ctggagacac ggctcaggac ggcgagctcc tggacacgtc tggcccgtac 1621 tcagagagct cgggcaccag cagccccagc acgtccccca gggcctccaa ccactcgctc 1681 tgctccggca gctctgcctc caaggctggc agcagcccct ccctggaaca agacgatgga 1741 gatgaggaaa ccagcgtggt gatagttggg aaaatttcct tctgtcccaa ggatgtcctg 1801 ggccatggag ctgagggcac aattgtgtac cggggcatgt ttgacaaccg cgacgtggcc 1861 gtgaagagga tcctccccga gtgttttagc ttcgcagacc gtgaggtcca gctgttgcga 1921 gaatcggatg agcacccgaa cgtgatccgc tacttctgca cggagaagga ccggcaattc 1981 cagtacattg ccatcgagct gtgtgcagcc accctgcaag agtatgtgga gcagaaggac 2041 tttgcgcatc tcggcctgga gcccatcacc ttgctgcagc agaccacctc gggcctggcc 2101 cacctccact ccctcaacat cgttcacaga gacctaaagc cacacaacat cctcatatcc 2161 atgcccaatg cacacggcaa gatcaaggcc atgatctccg actttggcct ctgcaagaag 2221 gtgggcagtg gggcaaacca agttttcagc cgcccgattt ggggggccct ggcacagaag 2281 gttggatcgt cccagagatg ctgagcgaag actgtaagga gaaccctacg tacacggtgg 2341 acatcttttt tgcaggttgc gtcttttact acgtaatctc tgagggcagc cacctttttg 2401 gcaagtccct gcagcggcag gccaacatcc tcctgggtgc ctgcagcctt gactgcttgc 2461 acccagagaa gcacgaagac gtcattgcac gtgaattgat agagaagatg attgcgatgg 2521 atcctcagaa acgcccctca gcgaagcacg tgctcaaaca cccgttcttc tggagcctag 2581 agaagcagct ccagttcttc caggacgtga gcgacagaat agaaaaggaa tccctggatg 2641 gcccgatcgt gaagcagtta gagagaggcg ggagagccgt ggtgaagatg gactggcggg 2701 agaacatcac tgtccccctc cagacagacc tgcgtaaatt caggacctat aaaggtggtt 2761 ctgtcagaga tctccTCCGA GCCATGAGAA ATAAGAAGCA CCACTACCGG GAGCTGCCTG 2821 CAGAGGTGCG GGAGACGCTG GGGTCCCTCC CCGACGACTT CGTGTGCTAC TTCACGTCTC 2881 GCTTCCCCCA CCTCCTCGCA CACACCTACC GGGCCATGGA GCTGTGCAGC CACGAGAGAC 2941 TCTTCCAGCC CTACTACTTC CACGAGCCCC CAGAGCCCCA GCCCCCAGTG ACTCCAGACG 3001 CCCTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3061 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3121 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCACACCT CTAGATATCT CACATACGCG 3181 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt