Construct: ORF TRCN0000465952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008179.1_s317c1
- Derived from:
- ccsbBroadEn_01481
- DNA Barcode:
- ACATTAGACTGTTTTTCCGTCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SARS1 (6301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6301 | SARS1 | seryl-tRNA synthetase 1 | NM_006513.4 | 100% | 100% | |
| 2 | human | 6301 | SARS1 | seryl-tRNA synthetase 1 | NM_001330669.1 | 95.8% | 95.8% | 1255_1320del |
| 3 | mouse | 20226 | Sars | seryl-aminoacyl-tRNA synthe... | NM_001204979.1 | 90.2% | 95.5% | (many diffs) |
| 4 | mouse | 20226 | Sars | seryl-aminoacyl-tRNA synthe... | NM_011319.3 | 86.2% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctggatctg gatttgtttc gggtggataa aggaggggac ccagccctca 121 tccgagagac gcaggagaag cgcttcaagg acccgggact agtggaccag ctggtgaagg 181 cagacagcga gtggcgacga tgtagatttc gggcagacaa cttgaacaag ctgaagaacc 241 tatgcagcaa gacaatcgga gagaaaatga agaaaaaaga gccagtggga gatgatgagt 301 ctgtcccaga gaatgtgctg agtttcgatg accttactgc agacgcttta gctaacctga 361 aagtctcaca aatcaaaaaa gtccgactcc tcattgatga agccatcctg aagtgtgacg 421 cggagcggat aaagttggaa gcagagcggt ttgagaacct ccgagagatt gggaaccttc 481 tgcacccttc tgtacccatc agtaacgatg aggatgtgga caacaaagta gagaggattt 541 ggggtgattg tacagtcagg aagaagtact ctcatgtgga cctggtggtg atggtagatg 601 gctttgaagg cgaaaagggg gccgtggtgg ctgggagtcg agggtacttc ttgaaggggg 661 tcctggtgtt cctggaacag gctctcatcc agtatgccct tcgcaccttg ggaagtcggg 721 gctacattcc catttatacc ccctttttca tgaggaagga ggtcatgcag gaggtggcac 781 agctcagcca gtttgatgaa gaactttata aggtgattgg caaaggcagt gaaaagtctg 841 atgacaactc ctatgatgag aagtacctga ttgccacctc agagcagccc attgctgccc 901 tgcaccggga tgagtggctc cggccggagg acctgcccat caagtatgct ggcctgtcta 961 cctgcttccg tcaggaggtg ggctcccatg gccgtgacac ccgtggcatc ttccgagtcc 1021 atcagtttga gaagattgaa cagtttgtgt actcatcacc ccatgacaac aagtcatggg 1081 agatgtttga agagatgatt accaccgcag aggagttcta ccagtccctg gggattcctt 1141 accacattgt gaatattgtc tcaggttctt tgaatcatgc tgccagtaag aagcttgacc 1201 tggaggcctg gtttccgggc tcaggagcct tccgtgagtt ggtctcctgt tctaattgca 1261 cggattacca ggctcgccgg cttcgaatcc gatatgggca aaccaagaag atgatggaca 1321 aggtggagtt tgtccatatg ctcaatgcta ccatgtgcgc cactacccgt accatcTGCG 1381 CCATCCTGGA GAACTACCAG ACAGAGAAGG GCATCACTGT GCCTGAGAAA TTGAAGGAGT 1441 TCATGCCGCC AGGACTGCAA GAACTGATCC CCTTTGTGAA GCCTGCGCCC ATTGAGCAGG 1501 AGCCATCAAA GAAGCAGAAG AAGCAACATG AGGGCAGCAA AAAGAAAGCA GCAGCAAGAG 1561 ACGTCACCCT AGAAAACAGG CTGCAGAACA TGGAGGTCAC CGATGCTTGC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGAACATTAG ACTGTTTTTC CGTCAATACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt