Construct: ORF TRCN0000465952

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008179.1_s317c1
Derived from:
ccsbBroadEn_01481
DNA Barcode:
ACATTAGACTGTTTTTCCGTCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SARS1 (6301)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465952
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6301 SARS1 seryl-tRNA synthetase 1 NM_006513.4 100% 100%
2 human 6301 SARS1 seryl-tRNA synthetase 1 NM_001330669.1 95.8% 95.8% 1255_1320del
3 mouse 20226 Sars seryl-aminoacyl-tRNA synthe... NM_001204979.1 90.2% 95.5% (many diffs)
4 mouse 20226 Sars seryl-aminoacyl-tRNA synthe... NM_011319.3 86.2% 91.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1608
ORF length:
1542
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gctggatctg gatttgtttc gggtggataa aggaggggac ccagccctca
121tccgagagac gcaggagaag cgcttcaagg acccgggact agtggaccag ctggtgaagg
181cagacagcga gtggcgacga tgtagatttc gggcagacaa cttgaacaag ctgaagaacc
241tatgcagcaa gacaatcgga gagaaaatga agaaaaaaga gccagtggga gatgatgagt
301ctgtcccaga gaatgtgctg agtttcgatg accttactgc agacgcttta gctaacctga
361aagtctcaca aatcaaaaaa gtccgactcc tcattgatga agccatcctg aagtgtgacg
421cggagcggat aaagttggaa gcagagcggt ttgagaacct ccgagagatt gggaaccttc
481tgcacccttc tgtacccatc agtaacgatg aggatgtgga caacaaagta gagaggattt
541ggggtgattg tacagtcagg aagaagtact ctcatgtgga cctggtggtg atggtagatg
601gctttgaagg cgaaaagggg gccgtggtgg ctgggagtcg agggtacttc ttgaaggggg
661tcctggtgtt cctggaacag gctctcatcc agtatgccct tcgcaccttg ggaagtcggg
721gctacattcc catttatacc ccctttttca tgaggaagga ggtcatgcag gaggtggcac
781agctcagcca gtttgatgaa gaactttata aggtgattgg caaaggcagt gaaaagtctg
841atgacaactc ctatgatgag aagtacctga ttgccacctc agagcagccc attgctgccc
901tgcaccggga tgagtggctc cggccggagg acctgcccat caagtatgct ggcctgtcta
961cctgcttccg tcaggaggtg ggctcccatg gccgtgacac ccgtggcatc ttccgagtcc
1021atcagtttga gaagattgaa cagtttgtgt actcatcacc ccatgacaac aagtcatggg
1081agatgtttga agagatgatt accaccgcag aggagttcta ccagtccctg gggattcctt
1141accacattgt gaatattgtc tcaggttctt tgaatcatgc tgccagtaag aagcttgacc
1201tggaggcctg gtttccgggc tcaggagcct tccgtgagtt ggtctcctgt tctaattgca
1261cggattacca ggctcgccgg cttcgaatcc gatatgggca aaccaagaag atgatggaca
1321aggtggagtt tgtccatatg ctcaatgcta ccatgtgcgc cactacccgt accatcTGCG
1381CCATCCTGGA GAACTACCAG ACAGAGAAGG GCATCACTGT GCCTGAGAAA TTGAAGGAGT
1441TCATGCCGCC AGGACTGCAA GAACTGATCC CCTTTGTGAA GCCTGCGCCC ATTGAGCAGG
1501AGCCATCAAA GAAGCAGAAG AAGCAACATG AGGGCAGCAA AAAGAAAGCA GCAGCAAGAG
1561ACGTCACCCT AGAAAACAGG CTGCAGAACA TGGAGGTCAC CGATGCTTGC CCAACTTTCT
1621TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1681CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1741GTGGAAAGGA CGAACATTAG ACTGTTTTTC CGTCAATACG CGTTAAGTCg acaatcaacc
1801tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)