Construct: ORF TRCN0000465979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005355.1_s317c1
- Derived from:
- ccsbBroadEn_04238
- DNA Barcode:
- CATCCCAGCCAACTCTTCTTAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf21 (81563)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465979
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81563 | C1orf21 | chromosome 1 open reading f... | NM_030806.4 | 100% | 100% | |
| 2 | mouse | 69399 | 1700025G04Rik | RIKEN cDNA 1700025G04 gene | NM_197990.3 | 93.9% | 96.6% | (many diffs) |
| 3 | mouse | 69399 | 1700025G04Rik | RIKEN cDNA 1700025G04 gene | XM_011248069.2 | 93.9% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgtgcctcc gccaagcatg ttgccactgt tcaaaatgaa gaggaagccc 121 agaaagggaa aaactaccag aacggagatg tgtttGGCGA TGAGTATAGG ATCAAACCAG 181 TGGAAGAGGT CAAATACATG AAAAATGGGG CAGAAGAAGA GCAGAAAATA GCAGCCAGGA 241 ACCAAGAAAA CTTGGAAAAA AGTGCCAGCT CAAATGTAAG ACTTAAAACT AATAAAGAGG 301 TTCCGGGATT AGTTCATCAA CCCAGAGCAA ACATGCACAT CTCTGAAAGC CAACAAGAAT 361 TCTTCAGAAT GCTGGATGAA AAAATTGAAA AGGGTCGGGA TTACTGTTCG GAAGAAGAGG 421 ATATCACATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATCCC AGCCAACTCT TCTTAGCAAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt