Construct: ORF TRCN0000465983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007136.1_s317c1
- Derived from:
- ccsbBroadEn_03701
- DNA Barcode:
- CACGGGCCCGTCCGTGATTTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGA9 (56107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56107 | PCDHGA9 | protocadherin gamma subfami... | NM_032089.1 | 100% | 100% | |
| 2 | human | 56107 | PCDHGA9 | protocadherin gamma subfami... | NM_018921.2 | 85.7% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2553
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagctcca accaaatgcc agctccgcgg aagattagtc ctgctatgct 121 cgctcctggg gatgctatgg gaggccaggg ccagtcagat tcgctactca gtgcctgaag 181 agacagaaaa gggctatatt gtgggcaaca tctccaagga cctggctctg gagccccggg 241 agctggcgga gcgccgagtc cgcatcgtct ctagaggtag gacgcagctt ttctctctga 301 acccgcgcag cggcaccttg gtcaccgcgg gtaggataga ccgggaggag ctctgtgctc 361 agagcccgcg gtgtctggtg aactttaaag tcctggttga agacagagtg aaactgtacg 421 gaatagaaat agaagtaact gatattaacg acagcgcccc aaagttccag gccgaaagtc 481 tggaagtaaa aattaacgaa atcgcggttc ctggagcacg ttatccactt ccagaagcta 541 ttgatccgga tgttggcgtg aactccctcc agagctacca gctcagcccc aatcaccact 601 tctccctgaa cgtgcagact ggagacaatg gagccataaa cccagagctg gtgctggagc 661 gcgccctgga cagggaggag gcaactgccc accacctggt cctcacggcc tcggatggcg 721 gcgagccgcg tcgctccagc acagtgcgca tccatgtgac agtgttggat acaaatgata 781 atgccccggt ttttgctcaa cggatttacc gagttaaagt ccttgagaac gtgcccccag 841 gcacctggct gcttactgca acagccagcg acctggatga gggaatcaac ggaaaagtgg 901 catacaaatt ctggaaaatt aatgaaaaac aatctctgct attccagctt aatgaaaata 961 ctggggaaat atcaacagca aaaagtctag attatgaaga atgttcattt tatgaaatgg 1021 aaatacaagc tgaagatggt gggggattga aagggtggac aaaagtgctc atttcggtgg 1081 aagatgtaaa tgacaataga cctgaagtga ccattacatc tctgtttagc ccagtgagag 1141 aagacgcacc tcagggaaca gtaattcttc ttttcaatgc tcatgaccga gactccggga 1201 agaatggtca agttgtctgt tctatccagg agaatctatc ttttacatta gaaaattcag 1261 aagaagatta ttacagattg ttgacggccc aaattcttga ccgagaaaaa gcctcagaat 1321 ataatatcac ggtgactgca acagacagag gaactccgcc cctgtccaca gaaattcaca 1381 tcaccctgca agtgactgac atcaatgata atccacctgc tttctctcaa gcctcctact 1441 cagtctacct cccggaaaac aacgccagag gtacttccat cttctccgtg attgcctatg 1501 accctgatag caatgagaat tctagagtta tttactcctt ggcagaggat accatccaag 1561 ggtctcctct ctccacctat gtctctatta actcagacac tggtgtgctg tatgctctgt 1621 gctcctttga ctatgagcag tttagagatt tgcaaatgca ggtgacggca agtgacagtg 1681 gaagcccacc acttagcagc aatgtgtcat tgagactgtt tgttttggac cagaatgaca 1741 atgccccaga aatcctgtac cctgccctcc ccactgatgg ttctactggt gtggagctgg 1801 caccccgctc tgcagagcct ggctacctgg tgaccaaggt ggtggcagtg gacagagact 1861 caggccagaa tgcttggctc tcctaccgcc tattcaaggc cagtgagcca gggctcttct 1921 cggtggggct gcacacaggt gaagtgcgca cagctcgggc cctgctagat agagatgcgc 1981 tcaaacagag ccttgtggtg gctgtacagg accatggcca gccccctctc tcggccactg 2041 tcacgctcac agtagccata gctgacagca tcccagacat cctggctgac ctgggcagtc 2101 ttcagatccc tgcagacctg gaggcctcag accttaccct ctacctcgtt gtggctgtgg 2161 cagtcgtctc ctgtgtcttc ctcaccttcg ttatcacgct gctggcccTC AGGCTGAGGC 2221 ACTGGCACTC CTCGCATCTG CTGCGGGCTA CCAGTGATGG GTTGGCTGGT GTGCCCACCT 2281 CACACTTTGT GGGTGTAGAT GGGGTTCGAG CTTTCCTACA GACCTATTCT CAGGAGTTCT 2341 CCCTCACCGC TGACTCAAGG AAGAGTCACC TGATCTTCCC CCAGCCCAAC TATGCAGACA 2401 CACTCATCAG CCAGCAGAGC TGTGAGAAAA ATGAGCCTTT GTGCGTCTCT GTTGATTCCA 2461 AGTTTCCTAT AGAAGACACC CCTTTGGTTC CGGTGAGTTC ATTTTTTTTC TTTCTTTCTT 2521 TTCTTTTTTT GTTTTTTGTT TTGTTTTGTT TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2581 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2641 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 2701 CGGGCCCGTC CGTGATTTCT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2761 ttgtgaaaga tt