Construct: ORF TRCN0000465990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003571.1_s317c1
- Derived from:
- ccsbBroadEn_00631
- DNA Barcode:
- CTTATAGGCCCCCTTGACCGTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFRA2 (2675)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465990
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | NM_001495.5 | 98.9% | 98.9% | 1378_1392del |
| 2 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | XM_006716327.3 | 98.9% | 98.9% | 1378_1392del |
| 3 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | XM_011544484.2 | 98.9% | 98.9% | 1378_1392del |
| 4 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | XM_024447127.1 | 83.6% | 83.6% | 0_1ins213;1165_1179del |
| 5 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | NM_001165038.2 | 76.2% | 76.2% | 39_40ins315;1063_1077del |
| 6 | human | 2675 | GFRA2 | GDNF family receptor alpha 2 | NM_001165039.2 | 69.2% | 68.3% | (many diffs) |
| 7 | mouse | 14586 | Gfra2 | glial cell line derived neu... | NM_008115.3 | 90.1% | 93.3% | (many diffs) |
| 8 | mouse | 14586 | Gfra2 | glial cell line derived neu... | NM_001302094.1 | 69.3% | 71.3% | (many diffs) |
| 9 | mouse | 14586 | Gfra2 | glial cell line derived neu... | NM_001302095.1 | 62.8% | 63.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1443
- ORF length:
- 1377
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cttggcaaac gtcttctgcc tcttcttctt tctagacgag accctccgct 121 ctttggccag cccttcctcc ctgcagggcc ccgagctcca cggctggcgc cccccagtgg 181 actgtgtccg ggccaatgag ctgtgtgccg ccgaatccaa ctgcagctct cgctaccgca 241 ctctgcggca gtgcctggca ggccgcgacc gcaacaccat gctggccaac aaggagtgcc 301 aggcggcctt ggaggtcttg caggagagcc cgctgtacga ctgccgctgc aagcggggca 361 tgaagaagga gctgcagtgt ctgcagatct actggagcat ccacctgggg ctgaccgagg 421 gtgaggagtt ctacgaagcc tccccctatg agccggtgac ctcccgcctc tcggacatct 481 tcaggcttgc ttcaatcttc tcagggacag gggcagaccc ggtggtcagc gccaagagca 541 accattgcct ggatgctgcc aaggcctgca acctgaatga caactgcaag aagctgcgct 601 cctcctacat ctccatctgc aaccgcgaga tctcgcccac cgagcgctgc aaccgccgca 661 agtgccacaa ggccctgcgc cagttcttcg accgggtgcc cagcgagtac acctaccgca 721 tgctcttctg ctcctgccaa gaccaggcgt gcgctgagcg ccgccggcaa accatcctgc 781 ccagctgctc ctatgaggac aaggagaagc ccaactgcct ggacctgcgt ggcgtgtgcc 841 ggactgacca cctgtgtcgg tcccggctgg ccgacttcca tgccaattgt cgagcctcct 901 accagacggt caccagctgc cctgcggaca attaccaggc gtgtctgggc tcttatgctg 961 gcatgattgg gtttgacatg acacctaact atgtggactc cagccccact ggcatcgtgg 1021 tgtccccctg gtgcagctgt cgtggcagcg ggaacatgga ggaggagtgt gagaagttcc 1081 tcagggactt caccgagaac ccatgccTCC GGAACGCCAT CCAGGCCTTT GGCAACGGCA 1141 CGGACGTGAA CGTGTCCCCA AAAGGCCCCT CGTTCCAGGC CACCCAGGCC CCTCGGGTGG 1201 AGAAGACGCC TTCTTTGCCA GATGACCTCA GTGACAGTAC CAGCTTGGGG ACCAGTGTCA 1261 TCACCACCTG CACGTCTGTC CAGGAGCAGG GGCTGAAGGC CAACAACTCC AAAGAGTTAA 1321 GCATGTGCTT CACAGAGCTC ACGACAAATA TCATCCCAGG GAGTAACAAG GTGATCAAAC 1381 CTAACTCAGG CCCCAGCAGA GCCAGACCGT CGGCTGCCTT GACCGTGCTG TCTGTCCTGA 1441 TGCTGAAACT GGCCTTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTATAG GCCCCCTTGA 1621 CCGTATGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt