Construct: ORF TRCN0000466018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013426.1_s317c1
- Derived from:
- ccsbBroadEn_09755
- DNA Barcode:
- CTTTGGTCGGCTCTAATGATCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNST (163882)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163882 | CNST | consortin, connexin sorting... | NM_001139459.1 | 99.9% | 99.8% | 260T>C |
| 2 | human | 163882 | CNST | consortin, connexin sorting... | NM_152609.3 | 84.7% | 84.4% | (many diffs) |
| 3 | human | 163882 | CNST | consortin, connexin sorting... | XM_005273081.3 | 84.7% | 84.4% | (many diffs) |
| 4 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544110.3 | 84.7% | 84.4% | (many diffs) |
| 5 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544111.1 | 84.7% | 84.4% | (many diffs) |
| 6 | human | 163882 | CNST | consortin, connexin sorting... | XM_017000487.1 | 67.7% | 67.1% | (many diffs) |
| 7 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544112.1 | 66% | 65.7% | (many diffs) |
| 8 | human | 163882 | CNST | consortin, connexin sorting... | XM_005273083.5 | 62% | 58.7% | (many diffs) |
| 9 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544113.2 | 62% | 58.7% | (many diffs) |
| 10 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544114.2 | 60.8% | 58.3% | (many diffs) |
| 11 | human | 163882 | CNST | consortin, connexin sorting... | XM_017000488.1 | 60.8% | 58.3% | (many diffs) |
| 12 | human | 163882 | CNST | consortin, connexin sorting... | XM_017000489.2 | 60.8% | 58.3% | (many diffs) |
| 13 | human | 163882 | CNST | consortin, connexin sorting... | XM_024453697.1 | 56% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacagcgat actcctacat attatctgca aatagaacca caagatggat 121 gtcatcctgg tgacagcgtg gaaaggagtg tgacctgtct gccttctgca tcagatgaaa 181 atgaaaatca gcttgacggg gacgggcatg agcatctgac cagcagtgac agtgcgatgg 241 gaaagcccca agtgtctgag caggacagtc tcaataataa tgaaagctgc acattgagct 301 gcgaggtggc tgcaggtgag aactcgcaaa acaccctttg tgaagcctcc agagatgaac 361 aggccttctt gggaaaggac aaaaaaattc ctggaaaaag aagtccaaga agcaaaaaag 421 ggactgctaa gaagatacca ccaggacttt tttcaggaga tattgcacct ttaatgcaag 481 aaaaagtact aagcgcagtc acatatgctg ttgatgatga agaagctgct gaagtaaatg 541 ctaatgagca gccagaggcg ccaaagcttg ttctgcagtc tctgttttca cttatacgag 601 gtgaagttga gcagttggat tcaagagcac ttcccctttg ccttcatcag atagcagaat 661 cctatttcca ggaggaggac tatgagaaag caatgaaatt cattcagcta gaacgattgt 721 atcatgagca attgctcgca aatctttctg ccattcaaga acagtgggaa acaaaatgga 781 aaactgtgca accacataca gttacggctc taaggaattc agaaaaggga tttaatggtg 841 aagattttga acggcttacg aaaatttgtg caacacatca agatcctctt ttatccaaac 901 ataagatagc agctgtggag aagtcccagg aaaggaaatg ctccacgcag ttactagtgt 961 ctgaagatcc aaaggaagga ggagctacca ccaaagagtc agagagtaaa acttgtctcg 1021 gcacagagtc aagtaaagaa agccaacata cagtggagcc cctggggagc agtccctgct 1081 gtcatcagat ggacgtgcaa acagattccc caagcctttc ggtaactgca ggaaaggacc 1141 acatggagga gctgctctgc agcgctgaag ccacgttagc gctccacacc cagtcctccg 1201 agacagcagg gagcccgtct gggccagact cttctgagga tgcttgtgag gatgacagtc 1261 gcttgcagct ggctcaaaca gaggcctgcc aggatgtggc cagaatagag ggcattgctg 1321 aagaccctaa ggtgtttctt tccagcaagt caaagacaga gccgttgatt tcaccaggct 1381 gtgaccgtat acctcctgca ttgatttctg agggtaaata ttcacaggct cagaggaaag 1441 aactccgttt gccacttcgg gatgcttctg aggcgttgcc cacagaccaa cttgagaaca 1501 atgaattaaa tgagctgcag cagcctgatc ttacagacag tgatggaaaa tcacCACAGG 1561 CGCAGGCTGA CTCAGACGGT TCTGAGAATG TGCTCTGTGG AAATAATCAA ATATCTGACT 1621 TAGGCATACT GCTTCCAGAG GTGTGTATGG CCCCAGAGGA AAAGGGAGAT AAAGACGACC 1681 AACTCAACAA AGAAACAGAA GACTATTTGA ACAGCCTTTT AGAAGGATGT TTAAAAGATA 1741 CTGAAGATTC CCTTTCCTAT GAAGATAACC AAGACGACGA CTCCGATCTC CTTCAAGATC 1801 TCTCTCCTGA AGAAGCATCC TATAGTCTCC AGGAGAATCT GCCTTCTGAT GAGAGCTGTC 1861 TTTCTCTTGA TGATCTTGCC AAAAGGATAG AGATTGCAGA GGTAAATCAG AGATGCCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAC TTTGGTCGGC TCTAATGATC AGAACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att