Construct: ORF TRCN0000466028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000446.1_s317c1
- Derived from:
- ccsbBroadEn_09005
- DNA Barcode:
- GAAAATACCAAAACAACTCGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC170 (80129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80129 | CCDC170 | coiled-coil domain containi... | NM_025059.4 | 99.8% | 99.7% | 991G>A;1317T>C;2047G>A |
| 2 | human | 80129 | CCDC170 | coiled-coil domain containi... | XM_011536147.2 | 97.5% | 96.8% | (many diffs) |
| 3 | human | 80129 | CCDC170 | coiled-coil domain containi... | XM_011536148.2 | 88.2% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2211
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctggactgc accagccata tcgcgctggg tgccgcttcg ccagcgcccg 121 aggaaactta cgatcatctt tcggaagtcc cggtcacgcg ggagcagtta aaccactatc 181 ggaatgtggc tcaaaatgct cgaagtgaac ttgcagcaac tttggtcaaa tttgaatgtg 241 ctcagtctga gcttcaagac ctccgatcca agatgctttc taaagaagtc tcctgtcaag 301 aactgaaagc tgaaatggag agctacaagg aaaacaatgc cagaaaatca tctctcctta 361 cctctttgag agacagagtt caggaactag aagaagaatc agcagcactt tccacttcta 421 aaatcagaac agaaatcaca gctcacgctg caatcaagga gaaccaggaa ttaaagaaga 481 aagttgtaga gttaaatgaa aaattacaaa agtgttcaaa agaaaatgag gagaataaga 541 aacaagtttc aaagaattgc aggaaacatg aggaatttct gactcaactg cgtgactgct 601 tggatccaga tgagaggaat gacaaggcat cagatgaaga tttaatttta aagcttagag 661 acctgcgcaa agaaaatgaa ttcgtgaaag gacaaattgt tattcttgaa gagactataa 721 atgtccatga gatggaagca aaagctagca gagaaacgat catgaggctg gcttcagaag 781 tcaacagaga gcagaaaaaa gctgcctcct gtactgaaga gaaagagaag ctgaaccagg 841 acctgctcag tgctgtagaa gcaaaagaag ctcttgaaag ggaagttaag atcttccaag 901 aaaggctgct tgctggccag caggtctggg atgcctcaaa gcaggaagtg agcctcctga 961 agaaaagctc ttctgagttg gagaagagtt tgaaggccag tcaggatgca gtcacaacct 1021 cacaaagcca gtacttctca tttagggaga aaatcacagc cctccttagg ggcagattga 1081 gcatgactgg gtccactgag gacaccattt tggagaagat tcgagaaatg gacagccggg 1141 aagaaagcag ggaccggatg gtctcccagc ttgaagccca aatatctgag cttgttgaac 1201 agttgggaaa ggagtctggg tttcaccaga aagctctcca gagggcccag aaagcagaga 1261 atatgttgga gactcttcag ggtcagctga cacacctgga ggcagagctg gtttctggag 1321 gtgttttgcg agacaacttg aattttgaga aacaaaaata tcttaaattt ctggatcagc 1381 tctctcagaa aatgaagttg gaccagatgg ctgccgaact tggctttgac atgcggctgg 1441 acgtggtttt agctcgaaca gagcagctgg ttcgtcttga gagcaatgca gtcattgaga 1501 acaagaccat tgcccacaat ttgcagagaa agctaaagac acagaaagag agactggaga 1561 gcaaagaatt acacatgagc ctcctccggc agaaaatagc ccagctggag gaggagaagc 1621 aggcacgcac ggccttggtg gttgagaggg acaacgcgca tcttaccatc aggaacttgc 1681 agaagaaggt ggagaggctg cagaaagagc tgaacacgtg tcgagacttg cacaccgagc 1741 tcaaagccaa actggccgac accaatgaac tgaagattaa aactttggaa cagactaaag 1801 ccattgaaga tctaaacaaa tccagagacc aactggagaa gatgaaggag aaagctgaga 1861 aaaagctcat gtctgtcaag tcagaactgg ataccacaga acatgaggct aaggagaata 1921 aagaaagggC CAGAAACATG ATAGAAGTGG TAACCAGTGA AATGAAGACA CTAAAAAAAT 1981 CTCTGGAAGA AGCAGAAAAG AGAGAAAAGC AGCTGGCAGA CTTCAGGGAG GTGGTGTCGC 2041 AGATGCTAGG CTTGAACGTG ACCAGCCTTG CTCTTCCTGA TTATGAAATC ATCAAGTGTC 2101 TTGAAAGATT GATCCATTCA CATCAGCATC ACTTTGTTAC CTGTGCCTGC CTCAAAGATG 2161 TGACTACTGG GCAAGAGAGG CACCCACAAG GCCATTTACA GCTTCTTCAT TGCCCAACTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGAGAAA ATACCAAAAC AACTCGCCCA ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt