Construct: ORF TRCN0000466030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002531.1_s317c1
- Derived from:
- ccsbBroadEn_07346
- DNA Barcode:
- CGAGTTCAGCGGCGGGAATCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOK2 (9046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9046 | DOK2 | docking protein 2 | NM_003974.4 | 99.8% | 100% | 786G>C;1161C>T |
| 2 | human | 9046 | DOK2 | docking protein 2 | XM_005273680.4 | 77% | 77.1% | 63_64ins282;504G>C;879C>T |
| 3 | human | 9046 | DOK2 | docking protein 2 | NM_001317800.2 | 62.4% | 62.6% | 0_1ins462;324G>C;699C>T |
| 4 | human | 9046 | DOK2 | docking protein 2 | NM_201349.3 | 62.4% | 62.6% | 0_1ins462;324G>C;699C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agacggggca gtgaaacaag gcttcttgta tcttcagcag cagcagacgt 121 ttggaaagaa atggcgccgc ttcggcgcct cactgtatgg agggtcggac tgcgccttgg 181 cccggctgga gctgcaggag ggcccggaga agcctcgtcg gtgtgaggct gcccggaagg 241 tcatccgcct cagtgactgc ctgcgggtgg ccgaggccgg cggagaggcc agcagccccc 301 gggacaccag tgccttcttc ctggagacca aggagcgcct gtacctcctg gcggcccctg 361 cagcggagcg cggcgactgg gtgcaggcca tctgcctcct ggccttcccc gggcagagga 421 aggagctctc ggggccagag ggaaagcaga gccggccctg catggaggaa aatgaattgt 481 acagcagcgc agtcacagtc ggcccccaca aggaatttgc tgtgaccatg agacctacag 541 aagccagtga gaggtgccac ctgcgggggt cctataccct ccgggctggg gagagtgccc 601 tggagctgtg gggtgggccc gagccaggga cccagctgta cgactggccc tacaggtttc 661 tgcggcgctt tgggcgggac aaggtaacct tttcctttga ggcaggccgt cgctgcgtct 721 ctggagaggg caactttgag ttcgaaaccc ggcaaggcaa tgagatcttc ttggccctgg 781 aagaggccat ctctgcccag aagaatgctg cacccgctac accccaaccg cagccagcca 841 caatccccgc ctcgctgccc cggcctgata gcccctactc tcggccgcat gactcactgc 901 cgccgccttc acccaccaca ccggtgccTG CTCCACGGCC TCGGGGCCAG GAGGGGGAGT 961 ATGCCGTGCC CTTCGATGCG GTGGCCCGTT CCTTGGGGAA GAACTTCAGG GGCATCTTGG 1021 CAGTCCCTCC TCAGCTCCTG GCCGACCCTC TGTACGACAG CATTGAGGAG ACCCTGCCCC 1081 CTCGACCTGA CCACATATAC GATGAGCCCG AGGGAGTGGC TGCCCTGTCC CTCTATGACA 1141 GCCCGCAGGA GCCCCGGGGT GAGGCATGGA GGAGGCAGGC GACAGCTGAC AGGGACCCTG 1201 CTGGCCTCCA GCATGTCCAG CCAGCTGGGC AGGATTTCTC TGCTTCTGGC TGGCAGCCAG 1261 GAACTGAGTA TGACAATGTT GTACTAAAGA AAGGCCCAAA GTGCCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGACGA GTTCAGCGGC GGGAATCTAA TACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t