Transcript: Human NM_003974.4

Homo sapiens docking protein 2 (DOK2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
DOK2 (9046)
Length:
1766
CDS:
90..1328

Additional Resources:

NCBI RefSeq record:
NM_003974.4
NBCI Gene record:
DOK2 (9046)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003974.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000166368 CGACCTGACCACATATACGAT pLKO.1 1107 CDS 100% 3.000 4.200 N DOK2 n/a
2 TRCN0000165539 GCTGATAAATTGCCTCTCCCA pLKO.1 1452 3UTR 100% 0.660 0.924 N DOK2 n/a
3 TRCN0000164202 CCAGGAACTGAGTATGACAAT pLKO.1 1281 CDS 100% 4.950 3.465 N DOK2 n/a
4 TRCN0000160872 GAACTGAGTATGACAATGTTG pLKO.1 1285 CDS 100% 4.950 3.465 N DOK2 n/a
5 TRCN0000164642 GCAAGGCAATGAGATCTTCTT pLKO.1 776 CDS 100% 4.950 3.465 N DOK2 n/a
6 TRCN0000165937 GCCAGGAACTGAGTATGACAA pLKO.1 1280 CDS 100% 4.950 3.465 N DOK2 n/a
7 TRCN0000165149 GCTTCCTGCACCTTTCAGATT pLKO.1 1716 3UTR 100% 4.950 3.465 N DOK2 n/a
8 TRCN0000165816 GTGACCATGAGACCTACAGAA pLKO.1 546 CDS 100% 4.950 3.465 N DOK2 n/a
9 TRCN0000162906 GAATTTGCTGTGACCATGAGA pLKO.1 537 CDS 100% 3.000 2.100 N DOK2 n/a
10 TRCN0000165885 GAGGGCAACTTTGAGTTCGAA pLKO.1 750 CDS 100% 3.000 2.100 N DOK2 n/a
11 TRCN0000162773 CAAGGAATTTGCTGTGACCAT pLKO.1 533 CDS 100% 2.640 1.848 N DOK2 n/a
12 TRCN0000164297 CTTCTTGTATCTTCAGCAGCA pLKO.1 116 CDS 100% 2.160 1.512 N DOK2 n/a
13 TRCN0000163668 GTTGTACTAAAGAAAGGCCCA pLKO.1 1302 CDS 100% 0.540 0.378 N DOK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003974.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07346 pDONR223 100% 99.8% 100% None 786G>C;1161C>T n/a
2 ccsbBroad304_07346 pLX_304 0% 99.8% 100% V5 786G>C;1161C>T n/a
3 TRCN0000466030 CGAGTTCAGCGGCGGGAATCTAAT pLX_317 30.5% 99.8% 100% V5 786G>C;1161C>T n/a
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