Construct: ORF TRCN0000466032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008156.1_s317c1
- Derived from:
- ccsbBroadEn_00817
- DNA Barcode:
- CCCCTTCTCACCGTGAGCAATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDH2 (3418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3418 | IDH2 | isocitrate dehydrogenase (N... | NM_002168.3 | 100% | 100% | |
| 2 | human | 3418 | IDH2 | isocitrate dehydrogenase (N... | NM_001289910.1 | 88.4% | 88.4% | 0_1ins156 |
| 3 | human | 3418 | IDH2 | isocitrate dehydrogenase (N... | NM_001290114.2 | 71.2% | 71.2% | 0_1ins390 |
| 4 | mouse | 269951 | Idh2 | isocitrate dehydrogenase 2 ... | NM_173011.2 | 90% | 95.1% | (many diffs) |
| 5 | mouse | 269951 | Idh2 | isocitrate dehydrogenase 2 ... | XM_006540911.3 | 64.4% | 67.4% | (many diffs) |
| 6 | mouse | 269951 | Idh2 | isocitrate dehydrogenase 2 ... | XM_006540912.2 | 45.1% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggctacctg cgggtcgtgc gctcgctctg cagagcctca ggctcgcggc 121 cggcctgggc gccggcggcc ctgacagccc ccacctcgca agagcagccg cggcgccact 181 atgccgacaa aaggatcaag gtggcgaagc ccgtggtgga gatggatggt gatgagatga 241 cccgtattat ctggcagttc atcaaggaga agctcatcct gccccacgtg gacatccagc 301 taaagtattt tgacctcggg ctcccaaacc gtgaccagac tgatgaccag gtcaccattg 361 actctgcact ggccacccag aagtacagtg tggctgtcaa gtgtgccacc atcacccctg 421 atgaggcccg tgtggaagag ttcaagctga agaagatgtg gaaaagtccc aatggaacta 481 tccggaacat cctggggggg actgtcttcc gggagcccat catctgcaaa aacatcccac 541 gcctagtccc tggctggacc aagcccatca ccattggcag gcacgcccat ggcgaccagt 601 acaaggccac agactttgtg gcagaccggg ccggcacttt caaaatggtc ttcaccccaa 661 aagatggcag tggtgtcaag gagtgggaag tgtacaactt ccccgcaggc ggcgtgggca 721 tgggcatgta caacaccgac gagtccatct caggttttgc gcacagctgc ttccagtatg 781 ccatccagaa gaaatggccg ctgtacatga gcaccaagaa caccatactg aaagcctacg 841 atgggcgttt caaggacatc ttccaggaga tctttgacaa gcactataag accgacttcg 901 acaagaataa gatctggtat gagcaccggc tcattgatga catggtggct caggtcctca 961 agtcttcggg tggctttgtg tgggcctgca agaactatga cggagatgtg cagtcagaca 1021 tcctggccca gggctttggc tcccttggcc tgatgacgtc cgtcctggtc tgccctgatg 1081 ggaagacgat tgaggctgag gccgctcatg ggaccgTCAC CCGCCACTAT CGGGAGCACC 1141 AGAAGGGCCG GCCCACCAGC ACCAACCCCA TCGCCAGCAT CTTTGCCTGG ACACGTGGCC 1201 TGGAGCACCG GGGGAAGCTG GATGGGAACC AAGACCTCAT CAGGTTTGCC CAGATGCTGG 1261 AGAAGGTGTG CGTGGAGACG GTGGAGAGTG GAGCCATGAC CAAGGACCTG GCGGGCTGCA 1321 TTCACGGCCT CAGCAATGTG AAGCTGAACG AGCACTTCCT GAACACCACG GACTTCCTCG 1381 ACACCATCAA GAGCAACCTG GACAGAGCCC TGGGCAGGCA GTACCCAACT TTCTTGTACA 1441 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1501 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1561 AGGACGACCC CTTCTCACCG TGAGCAATTG GACGCGTTAA GTCgacaatc aacctctgga 1621 ttacaaaatt tgtgaaagat t