Construct: ORF TRCN0000466048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012741.1_s317c1
- Derived from:
- ccsbBroadEn_06570
- DNA Barcode:
- TTTTAAGACAAAATGCATTTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCM5 (4174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4174 | MCM5 | minichromosome maintenance ... | NM_006739.4 | 99.9% | 100% | 831T>C;1707G>A |
| 2 | human | 4174 | MCM5 | minichromosome maintenance ... | XM_006724242.4 | 96.8% | 94% | (many diffs) |
| 3 | mouse | 17218 | Mcm5 | minichromosome maintenance ... | NM_001302540.1 | 88.2% | 95.6% | (many diffs) |
| 4 | mouse | 17218 | Mcm5 | minichromosome maintenance ... | NM_008566.3 | 88.2% | 95.6% | (many diffs) |
| 5 | mouse | 17218 | Mcm5 | minichromosome maintenance ... | XR_001778410.1 | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2268
- ORF length:
- 2202
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggattcgac gatcctggca ttttctacag cgacagcttc gggggcgacg 121 cccaggccga cgaggggcag gcccgcaaat cgcagctgca gaggcgcttc aaggagttcc 181 tgcggcagta ccgagtgggc accgaccgca cgggcttcac cttcaaatac agggatgaac 241 tcaagcggca ttacaacctg ggggagtact ggattgaggt ggagatggag gatctggcca 301 gctttgatga ggacctggcc gactacttgt acaagcagcc agccgagcac ctgcagctgc 361 tggaggaagc tgccaaggag gtagctgatg aggtgacccg gccccggcct tctggggagg 421 aggtgctcca ggacatccag gtcatgctca agtcggacgc cagcccttcc agcattcgta 481 gcctgaagtc ggacatgatg tcacacctgg tgaagatccc tggcatcatc atcgcggcct 541 ctgcggtccg tgccaaggcc acccgcatct ctatccagtg ccgcagctgc cgcaacaccc 601 tcaccaacat tgccatgcgc cctggcctcg agggctatgc cctgcccagg aagtgcaaca 661 cagatcaggc tgggcgcccc aaatgcccat tggacccgta cttcatcatg cccgacaaat 721 gcaaatgcgt ggacttccag accctgaagc tgcaggagct gcctgatgca gtcccccacg 781 gggagatgcc cagacacatg cagctctact gcgacaggta cctgtgtgac aaggtcgtcc 841 ctgggaacag ggttaccatc atgggcatct actccatcaa gaagtttggc ctgaccacca 901 gcaggggccg tgacagggtg ggcgtgggca tccgaagctc ctacatccgt gtcctgggca 961 tccaggtgga cacagatggc tctggccgca gctttgctgg ggccgtgagc ccccaggagg 1021 aggaggagtt ccgtcgcctg gctgccctcc caaatgtcta tgaggtcatc tccaagagca 1081 tcgccccctc catctttggg ggcacagaca tgaagaaggc cattgcctgc ctgctctttg 1141 ggggctcccg aaagaggctc cctgatggac ttactcgccg aggagacatc aacctgctga 1201 tgctagggga ccctgggaca gccaagtccc agcttctgaa gtttgtggag aagtgttctc 1261 ccattggggt atacacgtct gggaaaggca gcagcgcagc tggactgaca gcctcggtga 1321 tgagggaccc ttcgtcccgg aatttcatca tggagggcgg agccatggtc ctggccgatg 1381 gtggggtcgt ctgtattgac gagtttgaca agatgcgaga agatgaccgt gtggcaatcc 1441 acgaagccat ggagcagcag accatctcta tcgccaaggc tgggatcacc accaccctga 1501 actcccgctg ctccgtcctg gctgctgcca actcagtgtt cggccgctgg gatgagacga 1561 agggggagga caacattgac ttcatgccca ccatcttgtc gcgcttcgac atgatcttca 1621 tcgtcaagga tgagcacaat gaggagaggg atgtgatgct ggccaagcat gtcatcactc 1681 tgcacgtgag cgcactgaca cagacacagg ctgtggaggg cgagattgac ctggccaagc 1741 tgaagaagtt tattgcctac tgccgagtga aatgtggccc ccggctgtca gcagaggctg 1801 cagagaaact gaagaaccgc tacatcatca tgcggagcgg ggcccgtcag cacgagaggg 1861 acagtgaccg ccgctccagc atccccatca ctgtgcggca gctggaggcc attgtgcgca 1921 tcgcggaagc cctcagcaag atgaagctgc agcccTTCGC CACAGAGGCA GATGTGGAGG 1981 AGGCCCTGCG GCTCTTCCAA GTGTCCACGT TGGATGCTGC CTTGTCCGGT ACCCTGTCAG 2041 GGGTGGAGGG CTTCACCAGC CAGGAGGACC AGGAGATGCT GAGCCGCATC GAGAAGCAGC 2101 TCAAGCGCCG CTTTGCCATT GGCTCCCAGG TGTCTGAGCA CAGCATCATC AAGGACTTCA 2161 CCAAGCAGAA ATACCCGGAG CACGCCATCC ACAAGGTGCT GCAGCTCATG CTGCGGCGCG 2221 GCGAGATCCA GCATCGCATG CAGCGCAAGG TTCTCTACCG CCTCAAGTGC CCAACTTTCT 2281 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2341 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2401 GTGGAAAGGA CGATTTTAAG ACAAAATGCA TTTTCATACG CGTTAAGTCg acaatcaacc 2461 tctggattac aaaatttgtg aaagatt