Transcript: Mouse NM_001302540.1

Mus musculus minichromosome maintenance complex component 5 (Mcm5), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Mcm5 (17218)
Length:
3417
CDS:
98..2302

Additional Resources:

NCBI RefSeq record:
NM_001302540.1
NBCI Gene record:
Mcm5 (17218)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001302540.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000349880 TTGTATGTACAGAGGTAATAA pLKO_005 2606 3UTR 100% 15.000 21.000 N Mcm5 n/a
2 TRCN0000313276 ACTCACTCGCCGAGGTGATAT pLKO_005 1201 CDS 100% 13.200 18.480 N Mcm5 n/a
3 TRCN0000127210 CCTACCATCTTGTCCCGATTT pLKO.1 1619 CDS 100% 10.800 15.120 N Mcm5 n/a
4 TRCN0000312257 CCTACCATCTTGTCCCGATTT pLKO_005 1619 CDS 100% 10.800 15.120 N Mcm5 n/a
5 TRCN0000420069 GATGAACTCAAGCGGCATTAC pLKO_005 266 CDS 100% 10.800 15.120 N MCM5 n/a
6 TRCN0000127212 CCGCATTGAGAAGCAACTCAA pLKO.1 2116 CDS 100% 0.495 0.693 N Mcm5 n/a
7 TRCN0000312259 CCGCATTGAGAAGCAACTCAA pLKO_005 2116 CDS 100% 0.495 0.693 N Mcm5 n/a
8 TRCN0000127211 CCTTCCCAGGAAGTGCAATAT pLKO.1 673 CDS 100% 13.200 9.240 N Mcm5 n/a
9 TRCN0000417228 CATCCAGGTCATGCTCAAGTC pLKO_005 466 CDS 100% 4.050 2.835 N MCM5 n/a
10 TRCN0000127213 TGATATGATCTTCATCGTCAA pLKO.1 1639 CDS 100% 4.050 2.835 N Mcm5 n/a
11 TRCN0000312209 TGATATGATCTTCATCGTCAA pLKO_005 1639 CDS 100% 4.050 2.835 N Mcm5 n/a
12 TRCN0000127209 CCTGGAAATGTACTTTGGTCT pLKO.1 2427 3UTR 100% 2.640 1.848 N Mcm5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302540.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06570 pDONR223 100% 88.2% 95.6% None (many diffs) n/a
2 ccsbBroad304_06570 pLX_304 0% 88.2% 95.6% V5 (many diffs) n/a
3 TRCN0000466048 TTTTAAGACAAAATGCATTTTCAT pLX_317 14.5% 88.2% 95.6% V5 (many diffs) n/a
4 ccsbBroadEn_06569 pDONR223 100% 88.2% 95.5% None (many diffs) n/a
5 ccsbBroad304_06569 pLX_304 0% 88.2% 95.5% V5 (many diffs) n/a
6 TRCN0000470025 TATACGATAGGTCATCCACTATAT pLX_317 14.5% 88.2% 95.5% V5 (many diffs) n/a
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