Construct: ORF TRCN0000466061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012980.1_s317c1
- Derived from:
- ccsbBroadEn_07814
- DNA Barcode:
- CTACCCCATCTGTCTTCAGGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SACM1L (22908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | NM_014016.5 | 99.9% | 99.8% | 1301A>T |
| 2 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | NM_001319071.2 | 96.3% | 99.8% | 1301A>T;1762_1827del |
| 3 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | NM_001319072.2 | 89.5% | 78.5% | 0_1ins55;147_148ins128;1118A>T |
| 4 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | XM_011533500.2 | 89.5% | 78.5% | 0_1ins55;147_148ins128;1118A>T |
| 5 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | NM_001319073.2 | 82.3% | 82.2% | 0_1ins309;992A>T |
| 6 | human | 22908 | SACM1L | SAC1 like phosphatidylinosi... | XR_940392.3 | 45.3% | (many diffs) | |
| 7 | mouse | 83493 | Sacm1l | SAC1 suppressor of actin mu... | NM_030692.4 | 91% | 94.8% | (many diffs) |
| 8 | mouse | 83493 | Sacm1l | SAC1 suppressor of actin mu... | XM_017313703.1 | 81.7% | 75.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1827
- ORF length:
- 1761
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacggcggcc tacgagcagc tgaagctgca tatcacacct gaaaaatttt 121 atgtggaagc ttgtgatgat ggagcagatg acgtacttac cattgaccgt gtgtccacag 181 aggttaccct tgcagtcaag aaagatgttc ctccttcagc tgtcacaaga ccaatatttg 241 gtatactggg cacaatccat ctggtggcag gtaattatct tatagtcatt accaaaaaga 301 taaaagtagg tgaatttttc agtcatgtag tctggaaagc aacagatttt gatgtccttt 361 cttataagaa gacaatgttg cacttaactg atattcagtt acaagataat aaaaccttcc 421 tagcgatgct aaaccatgtc ttgaatgtgg atggatttta cttttcaaca acatatgatt 481 tgacccatac tttgcagcgg ctatccaaca ctagtcctga attccaagaa atgagtctct 541 tggaaagggc agatcagcgg tttgtatgga atggtcatct tctaagagaa ctttctgcac 601 agccagaggt tcatcggttt gcccttccag tgttacatgg ctttattacc atgcattcat 661 gttctattaa tggaaaatac tttgattgga ttctcatctc gaggaggagc tgtttcagag 721 ctggtgtgcg ctattatgta agaggaattg attcggaagg ccatgcagct aactttgtag 781 aaacagaaca aattgtgcac tacaatggga gcaaagcttc gtttgtacag actcgaggat 841 caatacctgt tttctggtcc caaagaccaa acctcaagta caaaccactg ccacagatca 901 gcaaagtagc aaatcacatg gacggtttcc aaaggcattt tgattcccaa gtaattattt 961 atggaaaaca agttataatc aatctgatta accagaaggg ctcggagaag ccacttgagc 1021 agacatttgc aacaatggtg tcttccttgg gaagtggaat gatgagatac attgcctttg 1081 acttccataa ggaatgtaaa aatatgagat gggatcgact aagtatttta ttggatcagg 1141 tagcagaaat gcaagatgaa ttaagttatt ttctagtgga ctctgctggc caggtggtgg 1201 caaaccagga aggcgtgttc cgaagcaatt gcatggattg tctagataga accaatgtga 1261 tccagagttt gttagctcgt cgttcacttc aggcccaact tcagagacta ggagttttgc 1321 atgtgggaca aaagcttgaa gaacaagatg aatttgagaa gattttcaaa aatgcctggg 1381 ctgacaacgc aaatgcttgt gcCAAGCAAT ATGCGGGAAC TGGTGCCTTG AAGACTGACT 1441 TTACCAGAAC TGGAAAGAGA ACTCATTTGG GACTTATAAT GGATGGCTGG AACTCAATGA 1501 TACGATATTA TAAGAACAAC TTTTCCGATG GATTTAGACA AGATTCCATA GACTTATTTC 1561 TTGGAAACTA TTCAGTGGAT GAATTAGAAT CTCATAGTCC TTTAAGTGTT CCAAGGGACT 1621 GGAAATTCCT GGCTTTGCCT ATTATCATGG TTGTTGCCTT TTCAATGTGC ATTATCTGTT 1681 TGCTTATGGC TGGTGACACT TGGACAGAAA CACTGGCCTA TGTGCTCTTC TGGGGAGTTG 1741 CAAGCATTGG AACATTTTTT ATCATTCTTT ACAATGGCAA AGATTTTGTC GATGCTCCCA 1801 GACTGGTCCA GAAAGAAAAG ATAGACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1861 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1921 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTACCCCA 1981 TCTGTCTTCA GGGTTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2041 aagatt