Construct: ORF TRCN0000466065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009211.1_s317c1
- Derived from:
- ccsbBroadEn_03834
- DNA Barcode:
- AGCTAACCTTCCAGCGCCAGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BEGAIN (57596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57596 | BEGAIN | brain enriched guanylate ki... | NM_001159531.1 | 100% | 100% | |
| 2 | human | 57596 | BEGAIN | brain enriched guanylate ki... | NM_020836.4 | 100% | 100% | |
| 3 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_005267921.3 | 100% | 100% | |
| 4 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_017021521.1 | 100% | 100% | |
| 5 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_005267920.5 | 98% | 98% | 1_36del |
| 6 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_005267919.4 | 97.8% | 97.8% | 1_39del |
| 7 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_011537031.1 | 96.8% | 96.8% | 1_57del |
| 8 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_024449669.1 | 94.5% | 95.1% | 1_93del;96_98delCTG;101_102insGAAACT |
| 9 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_024449670.1 | 94.5% | 95.1% | 1_93del;96_98delCTG;101_102insGAAACT |
| 10 | human | 57596 | BEGAIN | brain enriched guanylate ki... | XM_024449671.1 | 90% | 90.6% | (many diffs) |
| 11 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516044.2 | 85.4% | 86% | (many diffs) |
| 12 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_011244130.2 | 85.4% | 86% | (many diffs) |
| 13 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516043.3 | 83.6% | 84.2% | (many diffs) |
| 14 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516042.1 | 83.1% | 83.6% | (many diffs) |
| 15 | mouse | 380785 | Begain | brain-enriched guanylate ki... | NM_001163175.1 | 82.8% | 83.4% | (many diffs) |
| 16 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_011244128.2 | 81.5% | 81.9% | (many diffs) |
| 17 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516041.3 | 79.8% | 80.3% | (many diffs) |
| 18 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516040.3 | 79.3% | 79.8% | (many diffs) |
| 19 | mouse | 380785 | Begain | brain-enriched guanylate ki... | XM_006516039.2 | 79.1% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaactcagc gcgctgcagg agcagaaggg cgagctgcgc aagcggctgt 121 cctacaccac acacaagctc gagaagctcg agaccgagtt cgactccacg cgccactacc 181 tggagatcga gctgcggcgc gcgcaggagg aactggagaa ggtcacggag aagctgcgca 241 ggattcagag caactacatg gcactgcaga ggatcaacca ggagctggag gacaagctgt 301 accgcatggg ccagcactat gaggaggaga agcgtgcgct gagccacgag attgttgccc 361 tcaacagcca tctgctagaa gccaaggtga ccatcgacaa gctgtcagag gacaacgagc 421 tctataggaa ggactgcaat ctagcggccc agctgctgca gtgcagccag acctacggca 481 gggtccacaa ggtgtctgag ctgccctcgg atttccagga gcgcgtgagc ctgcacatgg 541 agaagcacgg ctgcagcctg ccatccccgc tctgccaccc ggcctacgcc gacagcgtcc 601 ccacctgcgt cattgccaag gtgctggaga agccggaccc cgccagcctg tcctcccgcc 661 tgtccgatgc ctccgcccgc gacctggcct tctgcgacgg ggtggagaaa ccaggcccgc 721 ggccccccta caagggagac atctactgca gtgacacagc cctctactgc ccggaggagc 781 ggcggcgaga ccggcggcct agcgtggacg cgcccgtgac cgacgtgggc ttcctgcggg 841 cccagaactc cactgacagc gcggccgagg aggaggagga ggccgaggcg gcggccttcc 901 cggcgggctt ccagcatgag gccttcccca gctacgcagg ctcactgccc acgtccagct 961 cctactccag cttcagcgcc acgtcggagg agaaggagca cgcgcaggcc agcacgctga 1021 ccgcgtcgca gcaggccatc tacctgaaca gccgcgacga gctcttcgac cgcaagccac 1081 ccgccaccac ctacgagggc agccctcgct ttgccaaggc cacggccgcg gtggcggccc 1141 cgctggaggc cgaagtggcc ccaggcttcg ggcggaccat gtcaccgtac ccggccgaga 1201 ccttccgctt cccggcctct ccgggtcccc agcaggccct gatgccccca aacctgtgga 1261 gcctgcgggc caagccgggg accgcccggc tccccgggga ggacatgagg ggccagtggc 1321 gtcccctgag cgtggaggac atcggcgcct actcctaccc cgtgagcgct gccggccgcg 1381 cctcaccctg cagcttctct gaacgctact acggcggggc cgggggcagc ccgggcaaga 1441 aggccgacgg ccgcgccagc ccgctctacg ccagctacaa ggccgacagc ttctccgagg 1501 gggacgacct ctcccagggc cacctggcag agccctgctt cctgcgggcg ggcggcgacc 1561 tgagccTCAG TCCCGGCCGC TCGGCTGACC CACTGCCCGG CTATGCACCC AGCGAGGGGG 1621 GGGACGGGGA CAGGCTCGGG GTGCAGCTGT GTGGGACCGC CAGCAGCCCT GAGCCCGAGC 1681 AGGGTTCCAG GGACTCCTTG GAGCCGAGCT CCATGGAGGC CTCCCCGGAA ATGCATCCTG 1741 CCGCCCGCCT CAGCCCCCAG CAGGCCTTTC CGCGGACTGG TGGCTCGGGG CTGAGCCGCA 1801 AGGACAGCCT CACCAAGGCC CAGCTCTACG GAACCTTGCT CAACTGCCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA AGCTAACCTT CCAGCGCCAG CCTAACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt