Transcript: Mouse XM_011244128.2

PREDICTED: Mus musculus brain-enriched guanylate kinase-associated (Begain), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Begain (380785)
Length:
2887
CDS:
192..2084

Additional Resources:

NCBI RefSeq record:
XM_011244128.2
NBCI Gene record:
Begain (380785)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011244128.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000363021 CGGAGGACAACGAGCTCTATA pLKO_005 622 CDS 100% 13.200 18.480 N Begain n/a
2 TRCN0000088557 CTGCAACTACTCGGAACGTTA pLKO.1 1607 CDS 100% 4.950 3.960 N Begain n/a
3 TRCN0000363020 CGTAAGGACAGTCTCACTAAA pLKO_005 2034 CDS 100% 13.200 9.240 N Begain n/a
4 TRCN0000362953 GCTCTCCTACACCACGCATAA pLKO_005 332 CDS 100% 10.800 7.560 N Begain n/a
5 TRCN0000363022 TAGACAAGGTGACGGAGAAAC pLKO_005 430 CDS 100% 10.800 7.560 N Begain n/a
6 TRCN0000037640 CAGGATTCAGAGCAACTACAT pLKO.1 455 CDS 100% 4.950 3.465 N BEGAIN n/a
7 TRCN0000327730 CAGGATTCAGAGCAACTACAT pLKO_005 455 CDS 100% 4.950 3.465 N BEGAIN n/a
8 TRCN0000088554 CCGTAAGGACAGTCTCACTAA pLKO.1 2033 CDS 100% 4.950 3.465 N Begain n/a
9 TRCN0000088556 CTAGACAAGGTGACGGAGAAA pLKO.1 429 CDS 100% 4.950 3.465 N Begain n/a
10 TRCN0000088555 CGACGAGTTCTTCAACCGCAA pLKO.1 1271 CDS 100% 2.160 1.512 N Begain n/a
11 TRCN0000088553 CCCACATGAAGGTCCTTCATT pLKO.1 2438 3UTR 100% 0.563 0.394 N Begain n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011244128.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03834 pDONR223 100% 81.5% 81.9% None (many diffs) n/a
2 ccsbBroad304_03834 pLX_304 0% 81.5% 81.9% V5 (many diffs) n/a
3 TRCN0000466065 AGCTAACCTTCCAGCGCCAGCCTA pLX_317 16% 81.5% 81.9% V5 (many diffs) n/a
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