Construct: ORF TRCN0000466077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005466.1_s317c1
- Derived from:
- ccsbBroadEn_04548
- DNA Barcode:
- GGCCGACCGACTCGATATCAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD44 (91526)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NM_153697.3 | 100% | 100% | |
| 2 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XR_923062.2 | 52.6% | 1_183del;1285_2092del | |
| 3 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NM_001367496.1 | 37% | 37% | 1102_2973del |
| 4 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NM_001195144.2 | 36.9% | 36.9% | 1102_2979del |
| 5 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XM_005246948.2 | 36.9% | 36.9% | 1102_2982del |
| 6 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XM_006712838.3 | 36.8% | 36.8% | 1102_2991del |
| 7 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XM_011512148.2 | 36.3% | 36.3% | 1102_3027del |
| 8 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XM_005246947.2 | 36.3% | 36.3% | 1102_3033del |
| 9 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NM_001367497.1 | 34.7% | 34.7% | 1102_3168del |
| 10 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NM_001367495.1 | 34.1% | 34.1% | 1102_3222del |
| 11 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XM_024453216.1 | 31.8% | 31.8% | 0_1ins75;1027_3147del |
| 12 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | XR_001739037.1 | 26.6% | 1_183del;1285_4129del | |
| 13 | human | 91526 | ANKRD44 | ankyrin repeat domain 44 | NR_160034.1 | 17.6% | 1_176del;1278_6223del | |
| 14 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | NM_001081433.3 | 33.2% | 36.2% | (many diffs) |
| 15 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | XM_006496073.3 | 32.6% | 35.6% | (many diffs) |
| 16 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | XM_006496071.3 | 31.2% | 34% | (many diffs) |
| 17 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | XM_006496070.3 | 30.7% | 33.5% | (many diffs) |
| 18 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | XM_011238538.2 | 28.7% | 31.3% | (many diffs) |
| 19 | mouse | 329154 | Ankrd44 | ankyrin repeat domain 44 | XM_006496074.1 | 24.6% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtgctcaaa ctcaccgacc agccaccatt ggttcaggca atcttcagcg 121 gtgatccaga ggagatccgg atgctcatcc ataaaactga agatgtgaat actctggatt 181 ctgagaaacg aacccctctt catgtggccg catttctggg agatgcagag atcattgaac 241 tcctgatttt gtcaggagct cgtgtaaatg ccaaggacaa catgtggctg actccactgc 301 accgggctgt tgcttccaga agtgaagaag cagtacaggt tttgattaag cactcagctg 361 atgtcaatgc aagggacaag aactggcaga cccctcttca tgtggcagca gccaacaagg 421 ctgtcaaatg tgcagaagtg atcattcccc tgctgagcag tgtcaatgtc tccgaccgag 481 gggggcgcac agccttgcac catgcggctc tgaacggcca cgtggagatg gtcaatttac 541 tcttggccaa aggggcaaat atcaatgcat ttgacaagaa ggaccggcgt gctctgcact 601 gggcagcata catgggccac ttggatgttg tagcattgct cattaaccat ggcgcagaag 661 tgacctgtaa ggataagaag ggttataccc ctctgcatgc tgcagcctcc aatggacaga 721 ttaatgttgt caagcatctc ctgaacctgg gggtggagat tgatgaaatc aatgtctatg 781 gaaatacagc gcttcacatc gccTGCTACA ATGGACAGGA TGCTGTGGTT AACGAGTTGA 841 TTGACTACGG TGCTAACGTG AACCAGCCAA ACAATAATGG GTTCACCCCT TTGCATTTTG 901 CTGCTGCCTC CACTCATGGT GCTTTGTGTC TTGAATTGTT AGTAAACAAC GGGGCAGATG 961 TTAACATTCA GAGTAAAGAT GGCAAAAGTC CACTGCACAT GACAGCTGTC CATGGAAGGT 1021 TCACACGGTC ACAGACCCTC ATTCAGAATG GAGGTGAAAT TGACTGTGTG GATAAGGACG 1081 GCAACACTCC TCTCCATGTG GCTGCAAGAT ACGGTCATGA GCTTTTGATT AACACCTTAA 1141 TAACCAGCGG AGCTGACACA GCCAAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCCGACC 1321 GACTCGATAT CAATGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt