Construct: ORF TRCN0000466110
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006970.1_s317c1
- Derived from:
- ccsbBroadEn_13948
- DNA Barcode:
- TACGGTTTCCTATGTTTGGATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS12 (6206)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466110
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6206 | RPS12 | ribosomal protein S12 | NM_001016.4 | 99.4% | 99.2% | 394A>G;396A>G |
| 2 | mouse | 20042 | Rps12 | ribosomal protein S12 | NM_011295.6 | 90.9% | 99.2% | (many diffs) |
| 3 | mouse | 237433 | Gm4925 | predicted gene 4925 | NM_001037166.2 | 86.4% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaggaaggc attgctgctG GAGGTGTAAT GGACGTTAAT ACTGCTTTAC 121 AAGAGGTTCT GAAGACTGCC CTCATCCACG ATGGCCTAGC ACGTGGAATT CGCGAAGCTG 181 CCAAAGCCTT AGACAAGCGC CAAGCCCATC TTTGTGTGCT TGCATCCAAC TGTGATGAGC 241 CTATGTATGT CAAGTTGGTG GAGGCCCTTT GTGCTGAACA CCAAATCAAC CTAATTAAGG 301 TTGATGACAA CAAGAAACTA GGAGAATGGG TAGGCCTTTG TAAAATTGAC AGAGAGGGGA 361 AACCCCGTAA AGTGGTTGGT TGCAGTTGTG TAGTAGTTAA GGACTATGGC AAGGAGTCTC 421 AGGCCAAGGA TGTCATTGAA GAGTATTTCA AATGCAAGGA GTGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGATAC GGTTTCCTAT GTTTGGATCT TACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t