Construct: ORF TRCN0000466115
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001931.1_s317c1
- Derived from:
- ccsbBroadEn_05957
- DNA Barcode:
- GAACGAAATCACACAGCTCGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD9 (928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466115
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 928 | CD9 | CD9 molecule | NM_001769.4 | 99.8% | 100% | 6G>C |
| 2 | human | 928 | CD9 | CD9 molecule | NM_001330312.2 | 69.7% | 69.7% | 0_1ins207 |
| 3 | human | 928 | CD9 | CD9 molecule | XM_005253814.4 | 68.3% | 58.5% | (many diffs) |
| 4 | mouse | 12527 | Cd9 | CD9 antigen | NM_007657.3 | 85% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgtcaaagga ggcaccaagt gcatcaaata cctgctgttc ggatttaact 121 tcatcttctg gcttgccggg attgctgtcc ttgccattgg actatggctc cgattcgact 181 ctcagaccaa gagcatcttc gagcaagaaa ctaataataa taattccagc ttctacacag 241 gagtctatat tctgatcgga gccggcgccc tcatgatgct ggtgggcttc ctgggctgct 301 gcggggctgt gcaggagtcc CAGTGCATGC TGGGACTGTT CTTCGGCTTC CTCTTGGTGA 361 TATTCGCCAT TGAAATAGCT GCGGCCATCT GGGGATATTC CCACAAGGAT GAGGTGATTA 421 AGGAAGTCCA GGAGTTTTAC AAGGACACCT ACAACAAGCT GAAAACCAAG GATGAGCCCC 481 AGCGGGAAAC GCTGAAAGCC ATCCACTATG CGTTGAACTG CTGTGGTTTG GCTGGGGGCG 541 TGGAACAGTT TATCTCAGAC ATCTGCCCCA AGAAGGACGT ACTCGAAACC TTCACCGTGA 601 AGTCCTGTCC TGATGCCATC AAAGAGGTCT TCGACAATAA ATTCCACATC ATCGGCGCAG 661 TGGGCATCGG CATTGCCGTG GTCATGATAT TTGGCATGAT CTTCAGTATG ATCTTGTGCT 721 GTGCTATCCG CAGGAACCGC GAGATGGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAACG 901 AAATCACACA GCTCGGCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt