Construct: ORF TRCN0000466145

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015881.1_s317c1
Derived from:
ccsbBroadEn_15985
DNA Barcode:
GTACACCCTACCGTCGCATACGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARSJ (79642)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466145
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79642 ARSJ arylsulfatase family member J XM_017008597.2 19.9% 3% 1_775del;1031_1281del
2 human 79642 ARSJ arylsulfatase family member J XM_017008598.2 19.9% 3% 1_775del;1031_1281del
3 human 79642 ARSJ arylsulfatase family member J XM_017008599.2 19.9% 3% 1_775del;1031_1281del
4 human 79642 ARSJ arylsulfatase family member J XM_017008600.1 19.9% 3% 1_775del;1031_1281del
5 human 79642 ARSJ arylsulfatase family member J NM_001354211.2 17.7% 1.2% 1_943del;1199_1440del
6 human 79642 ARSJ arylsulfatase family member J XM_011532239.2 17.5% 1.2% 1_943del;1199_1449del
7 human 79642 ARSJ arylsulfatase family member J XM_017008593.2 17.5% 1.2% 1_943del;1199_1449del
8 human 79642 ARSJ arylsulfatase family member J XM_017008594.2 17.5% 1.2% 1_943del;1199_1449del
9 human 79642 ARSJ arylsulfatase family member J XM_017008595.2 17.5% 1.2% 1_943del;1199_1449del
10 human 79642 ARSJ arylsulfatase family member J XM_024454216.1 17.5% 1.2% 1_943del;1199_1449del
11 human 79642 ARSJ arylsulfatase family member J XM_024454215.1 17.3% 3.6% 1_970del;1226_1467del
12 human 79642 ARSJ arylsulfatase family member J XM_011532238.3 17.2% 3.6% 1_970del;1226_1476del
13 human 79642 ARSJ arylsulfatase family member J NM_001354210.2 14.2% 3% 1_1291del;1547_1788del
14 human 79642 ARSJ arylsulfatase family member J NM_024590.4 14.1% 3% 1_1291del;1547_1797del
15 human 79642 ARSJ arylsulfatase family member J XM_017008592.2 14.1% 3% 1_1291del;1547_1797del
16 human 79642 ARSJ arylsulfatase family member J XM_024454214.1 14.1% 3% 1_1291del;1547_1797del
17 mouse 271970 Arsj arylsulfatase J NM_173451.3 12.8% 3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
345
ORF length:
255
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTCCTGG GCAGCAGGCT ATGGGATCTG
121GAACACTGCA ATCCAGTCAG CCATCAGAGT GCAGCACTGG AAATTGCTTA CAGGAAATCC
181TGGCTACAGC GACTGGGTCC CCCCTCAGTC TTTCAGCAAC CTGGGACCGA ACCGGTGGCA
241CAATGAACGG ATCACCTTGT CAACTGGCAA AAGTGTATGG CTTTTCAACA TCACAGCCGA
301CCCATATGAG AGGGTGGACC TATCTAACAG GTATCCAGGA ATCGTGCCCA ACTTTCTTGT
361ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
421AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
481GAAAGGACGA GTACACCCTA CCGTCGCATA CGACACGCGT TAAGTCgaca atcaacctct
541ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)