Construct: ORF TRCN0000466149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000803.1_s317c1
- Derived from:
- ccsbBroadEn_09198
- DNA Barcode:
- ACATCACGTACCTGTTAGATCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOPX (84525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466149
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84525 | HOPX | HOP homeobox | NM_001145459.1 | 99.5% | 98.6% | 215C>T |
| 2 | human | 84525 | HOPX | HOP homeobox | NM_139211.4 | 99.5% | 98.6% | 215C>T |
| 3 | human | 84525 | HOPX | HOP homeobox | NM_139212.3 | 99.5% | 98.6% | 215C>T |
| 4 | human | 84525 | HOPX | HOP homeobox | XM_017008730.2 | 99.5% | 98.6% | 215C>T |
| 5 | human | 84525 | HOPX | HOP homeobox | XM_017008731.2 | 99.5% | 98.6% | 215C>T |
| 6 | human | 84525 | HOPX | HOP homeobox | XM_017008732.1 | 99.5% | 98.6% | 215C>T |
| 7 | human | 84525 | HOPX | HOP homeobox | XM_017008733.1 | 99.5% | 98.6% | 215C>T |
| 8 | human | 84525 | HOPX | HOP homeobox | XM_017008734.1 | 99.5% | 98.6% | 215C>T |
| 9 | human | 84525 | HOPX | HOP homeobox | XM_017008728.1 | 82.5% | 81.8% | 1_45del;260C>T |
| 10 | human | 84525 | HOPX | HOP homeobox | NM_032495.6 | 79.8% | 79.1% | 1_54del;269C>T |
| 11 | human | 84525 | HOPX | HOP homeobox | XM_017008729.2 | 65.4% | 64.8% | 1_114del;329C>T |
| 12 | human | 84525 | HOPX | HOP homeobox | XM_006714052.2 | 56.3% | 48.6% | (many diffs) |
| 13 | human | 84525 | HOPX | HOP homeobox | NM_001145460.1 | 55.8% | 48.6% | (many diffs) |
| 14 | mouse | 74318 | Hopx | HOP homeobox | NM_001159900.1 | 89% | 90.4% | (many diffs) |
| 15 | mouse | 74318 | Hopx | HOP homeobox | NM_001159901.1 | 89% | 90.4% | (many diffs) |
| 16 | mouse | 74318 | Hopx | HOP homeobox | NM_175606.3 | 89% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 212
- ORF end:
- 431
- ORF length:
- 219
- Sequence:
-
1 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 61 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 121 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 181 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGTCGGCG GAGACCGCGA GCGGCCCCAC 241 AGAGGACCAG GTGGAAATCC TGGAGTACAA CTTCAACAAG GTCGACAAGC ACCCGGATTC 301 CACCACGCTG TGCCTCATCG CGGCCGAGGC AGGCCTTTCC GAGGAGGAGA CCCAGAAATG 361 GTTTAAGCAG CGCCTGGCAA AGTGGCGGCG CTCAGAAGGC CTGCCCTCAG AGTGCAGATC 421 CGTCATAGAC AACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 481 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 541 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAT CACGTACCTG TTAGATCTTT 601 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt