Construct: ORF TRCN0000466151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000294.1_s317c1
- Derived from:
- ccsbBroadEn_04581
- DNA Barcode:
- GATGTAGCGCAATTCGGCTTTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP4C (92421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466151
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92421 | CHMP4C | charged multivesicular body... | NM_152284.4 | 100% | 100% | |
| 2 | mouse | 66371 | Chmp4c | charged multivesicular body... | NM_025519.2 | 82.7% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgtcatgag caagttgggc aagttcttta aagggggcgg ctcttctaag agccgagccg 121 ctcccagtcc ccaggaggcc ctggtccgac ttcgggagac tgaggagatg ctgggcaaga 181 aacaagagta cctggaaaat cgaatccaga gagaaatcgc cctggccaag aagcacggca 241 cgcagaataa gcgagctgca ttacaggcac taaagagaaa gaagaggttc gagaaacagc 301 tcactcagat tgatggcaca ctttctacca ttGAGTTCCA GAGAGAAGCC CTGGAGAACT 361 CACACACCAA CACTGAGGTG TTGAGGAACA TGGGCTTTGC AGCAAAAGCG ATGAAATCTG 421 TTCATGAAAA CATGGATCTG AACAAAATAG ATGATTTGAT GCAAGAGATC ACAGAGCAAC 481 AGGATATCGC CCAAGAAATC TCAGAAGCAT TTTCTCAACG GGTTGGCTTT GGTGATGACT 541 TTGATGAGGA TGAGTTGATG GCAGAACTTG AAGAATTGGA ACAGGAGGAA TTAAATAAGA 601 AGATGACAAA TATCCGCCTT CCAAATGTGC CTTCCTCTTC TCTCCCAGCA CAGCCAAATA 661 GAAAACCAGG CATGTCGTCC ACTGCACGTC GATCCCGAGC AGCATCTTCC CAGAGGGCAG 721 AAGAAGAGGA TGATGATATC AAACAATTGG CAGCTTGGGC TACCTACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA GATGTAGCGC AATTCGGCTT TTCAACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt