Construct: ORF TRCN0000466163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012004.3_s317c1
- Derived from:
- ccsbBroadEn_15002
- DNA Barcode:
- TCCGGCTGGTTATAACCAGTAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAST1 (22983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22983 | MAST1 | microtubule associated seri... | NM_014975.3 | 67.7% | 67.7% | 1_1521del |
| 2 | mouse | 56527 | Mast1 | microtubule associated seri... | XM_017312921.1 | 67.9% | 72.9% | (many diffs) |
| 3 | mouse | 56527 | Mast1 | microtubule associated seri... | XM_006531217.3 | 57.6% | 62.4% | (many diffs) |
| 4 | mouse | 56527 | Mast1 | microtubule associated seri... | XM_006531216.3 | 57.5% | 62.4% | (many diffs) |
| 5 | mouse | 56527 | Mast1 | microtubule associated seri... | NM_019945.2 | 57.4% | 62.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3255
- ORF length:
- 3189
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcacatcaag ctcacagatt tcggcctctc caagatgggg ctcatgagcc 121 tcaccaccaa cttatatgaa ggccacatcg agaaggacgc ccgagagttc ctggacaaac 181 aggtgtgtgg gaccccagag tacatcgcgc ccgaggtcat cctgcgtcaa ggctacggca 241 agccagtgga ctggtgggct atggggatca tcctctacga gttcctggtg ggctgtgtgc 301 ccttcttcgg agacacacca gaggagctat ttggacaggt catcagtgat gacatcctgt 361 ggcccgaggg ggatgaggcc ctacctacgg aggcccaact cctcatatcc agcctcctgc 421 agaccaaccc tctggtcagg cttggggcag gcggcgcttt tgaggtgaag cagcacagtt 481 tctttcgaga cctggactgg acagggctgc tgaggcagaa ggccgagttc atcccccacc 541 tagagtcgga agatgacact agctactttg acacccgctc agacaggtat caccacgtga 601 actcctatga cgaggatgac acgacggagg aggagcccgt ggaaatccgc cagttctctt 661 cctgctctcc gcgcttcagc aaggtgtata gcagcatgga gcagctgtcg cagcacgagc 721 ccaagacccc agtagcagct gcagggagca gcaagcggga gccgagcacc aagggccccg 781 aggagaaggt ggccggcaag cgggaggggc tgggcggcct gaccctgcgt gagaagacct 841 ggagaggggg ctctccggag atcaagcgat tctccgcgtc cgaggccagt ttcctggagg 901 gagaggccag tccccctttg ggcgcccgcc gccgtttctc ggcgctgctg gagcccagcc 961 gcttcagcgc cccccaagag gacgaggatg aggcccggct gcgcaggcct ccccggccca 1021 gctccgaccc cgcgggatcc ctggatgcac gggcccccaa agaggagact caaggggaag 1081 gcacctccag cgccggggac tccgaggcca ctgaccgtcc acgcccaggt gacctctgcc 1141 caccctcgaa ggatggggat gcatcaggcc caagggctac caatgacttg gttctgcgcc 1201 gggcgcggca ccagcagatg tcaggggatg tggcagtaga gaagaggcct tctcgaactg 1261 ggggcaaagt catcaaatca gcctcagcca ctgccttatc tgtcatgatt cctgcagtgg 1321 acccacatgg aagttcaccc cttgctagtc ccatgtctcc acgatctctg tcctccaacc 1381 catcctcacg ggactcctca cccagccggg actactcacc agctgtcagt gggctccgct 1441 cccccatcac catccagcgc tcgggcaaga agtatggctt cacactgcgt gccatccgtg 1501 tctacatggg tgacacggat gtctatagtg tccaccacat tgtctggcat gtggaggaag 1561 gaggcccagc ccaggaggca ggactctgtg ctggggacct catcacccac gtgaatgggg 1621 agcctgtgca tggcatggtg catcctgagg tcgtggagct gatccttaag agtggcaaca 1681 aggtagcagt gaccacaacg cccttcgaaa atacctctat ccgcattggt cccgcaaggc 1741 gcagcagcta caaggctaaa atggctcgga ggaacaagcg accctccgcc aaggagggcc 1801 aggagagcaa gaagcgcagc tccctcttcc ggaagatcac gaagcagtcg aacctgctgc 1861 atactagccg ctcgctgtcg tcgctgaacc gctcgctgtc atccagcgat agtctcccgg 1921 gctcgcctac gcacgggctg ccggcgcgct cgcccacgca cagctaccgc tccacgcctg 1981 actccgccta cctaggcgcc tcatcccaga gcagctcccc agcctcgagc acgcccaact 2041 cgcctgcgtc gtcggcgtcg caccacattc ggcccagcac gctgcacgga ctgtcgccaa 2101 agctccatcg ccagtaccgc tctgcgcgat gcaagtcggc cggcaacatc cctctatcgc 2161 cgctggcaca cacgccgtcc cccacgcagg cgtcaccgcc gccactgccg ggccacacgg 2221 tgggcagctc gcacactact cagagcttcc cggccaaact gcactcatcg cctcccgtcg 2281 tgcgcccgcg ccccaagagt gccgagcccc ctcgctcgcc gctcctcaag cgcgtgcagt 2341 cggccgagaa gctgggagcc tctttgagtg cggacaagaa gggcgcgctg cgcaaacaca 2401 gcctcgaggt gggccacccg gatttccgca aggacttcca tggcgagctg gcgctgcata 2461 gccttgccga gtccgacggt gagacgcccc cagtcgaggg ccttggcgcg ccccggcagg 2521 tcgccgtccg ccgcctgggc cgacaggagt cacctttgag cctgggcgcg gacccgttgc 2581 tgcccgaggg tgcctccagg ccaccagtgt cgagcaagga gaaggaatcc ccggggggcg 2641 ccgaggcgtg caccccaccc cgcgcgacga cccccggtgg ccggaccctg gagcgggacg 2701 tcggctgcac gcggcatcag agcgtgcaga cggaggatgg cactggcggg atggccaggg 2761 ctgtggccaa ggcggcgctg agcccggtgc aggaacacga gacaggccgg cgcagcagct 2821 ctggcgaggc gggcacaccc ctggtaccca ttgtcgtaga gcctgcgcgg cccggggcta 2881 aggctgtggt gcctcagcct ctgggcgcgg actccaaggg gttgcaggaa cccgcacccc 2941 tggcgccTTC CGTGCCCGAG GCCCCCCGGG GCCGGGAGCG CTGGGTGTTG GAGGTGGTGG 3001 AGGAGCGCAC CACGCTGAGC GGTCCTCGCT CCAAGCCCGC CTCCCCAAAG CTCTCCCCGG 3061 AGCCCCAGAC ACCCTCCCTA GCCCCAGCGA AGTGCAGTGC ACCCAGCAGT GCAGTGACCC 3121 CAGTCCCACC CGCATCCCTC TTGGGCTCAG GCACCAAGCC TCAAGTGGGG CTGACCTCCC 3181 GGTGCCCTGC TGAAGCTGTG CCCCCAGCAG GCCTGACCAA AAAAGGAGTG TCCAGTCCCG 3241 CACCCCCGGG ACCATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3301 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3361 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCGGCTGGT TATAACCAGT 3421 AAGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt