Transcript: Human NM_014975.3

Homo sapiens microtubule associated serine/threonine kinase 1 (MAST1), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
MAST1 (22983)
Length:
4853
CDS:
59..4771

Additional Resources:

NCBI RefSeq record:
NM_014975.3
NBCI Gene record:
MAST1 (22983)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145937 TGGTGCCATAGCCAGATGAA pXPR_003 GGG 287 6% 4 0.5661 MAST1 MAST1 76122
2 BRDN0001149113 GAGCTTGATGTGACCCATGG pXPR_003 AGG 1522 32% 14 0.5553 MAST1 MAST1 76121
3 BRDN0001148956 CTTCACCAGCTGAGTAACGA pXPR_003 AGG 811 17% 8 0.064 MAST1 MAST1 76124
4 BRDN0001148834 TGACGAGGATGACACGACGG pXPR_003 AGG 2080 44% 18 -0.2133 MAST1 MAST1 76123
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014975.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196385 GTGAAGAAGTTGCTTATTATC pLKO.1 881 CDS 100% 13.200 18.480 N MAST1 n/a
2 TRCN0000021544 CGTGATGATGAATCACGTCTA pLKO.1 583 CDS 100% 0.405 0.567 N MAST1 n/a
3 TRCN0000199373 CTGGTACCCATTGTCGTAGAG pLKO.1 4355 CDS 100% 0.000 0.000 N MAST1 n/a
4 TRCN0000199586 GCGCTTCAGCAAGGTGTATAG pLKO.1 2185 CDS 100% 10.800 8.640 N MAST1 n/a
5 TRCN0000232040 GCGCTTCAGCAAGGTGTATAG pLKO_005 2185 CDS 100% 10.800 8.640 N MAST1 n/a
6 TRCN0000232037 TGGTGAAGAAGTTGCTTATTA pLKO_005 879 CDS 100% 15.000 10.500 N MAST1 n/a
7 TRCN0000358271 ACACACCAGAGGAGCTATTTG pLKO_005 1827 CDS 100% 13.200 9.240 N MAST1 n/a
8 TRCN0000358269 AGGAGAAGCTGCGCGACTTTA pLKO_005 639 CDS 100% 13.200 9.240 N MAST1 n/a
9 TRCN0000257225 GTGCTAGCCCTGGAGTATTTG pLKO_005 1502 CDS 100% 13.200 9.240 N MAST1 n/a
10 TRCN0000232038 TCGATACCATCAAGCTCATAA pLKO_005 1179 CDS 100% 13.200 9.240 N MAST1 n/a
11 TRCN0000232039 TGAGCCTCACCACCAACTTAT pLKO_005 1629 CDS 100% 13.200 9.240 N MAST1 n/a
12 TRCN0000358270 ACGAGATCGTGATGATGAATC pLKO_005 576 CDS 100% 10.800 7.560 N MAST1 n/a
13 TRCN0000021546 CGCAGCAGCTACAAGGCTAAA pLKO.1 3254 CDS 100% 10.800 7.560 N MAST1 n/a
14 TRCN0000199760 GTCGTGGAGCTGATCCTTAAG pLKO.1 3164 CDS 100% 10.800 7.560 N MAST1 n/a
15 TRCN0000021545 CCACGGTCTACTTCTATGAAT pLKO.1 780 CDS 100% 5.625 3.938 N MAST1 n/a
16 TRCN0000021547 CCACTTCCTCTCCAAACACTT pLKO.1 448 CDS 100% 4.950 3.465 N MAST1 n/a
17 TRCN0000021548 GCACAGTTTCTTTCGAGACCT pLKO.1 1987 CDS 100% 2.640 1.848 N MAST1 n/a
18 TRCN0000199538 GCCTTTGTGGAGCGCGATATC pLKO.1 1313 CDS 100% 3.600 2.160 N MAST1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014975.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11656 pDONR223 100% 67.7% 67.7% None 1_1521del n/a
2 ccsbBroad304_11656 pLX_304 0% 67.7% 67.7% V5 1_1521del n/a
3 TRCN0000466906 AACAGACCGCCGCTGATACATACG pLX_317 11.4% 67.7% 67.7% V5 1_1521del n/a
4 ccsbBroadEn_15002 pDONR223 0% 67.7% 67.7% None 1_1521del n/a
5 ccsbBroad304_15002 pLX_304 0% 67.7% 67.7% V5 1_1521del n/a
6 TRCN0000466163 TCCGGCTGGTTATAACCAGTAAGC pLX_317 9.9% 67.7% 67.7% V5 1_1521del n/a
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