Construct: ORF TRCN0000466170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012077.1_s317c1
- Derived from:
- ccsbBroadEn_05364
- DNA Barcode:
- TGGCAGACGGTATGTAAAAATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC24A5 (283652)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283652 | SLC24A5 | solute carrier family 24 me... | NM_205850.3 | 100% | 100% | |
| 2 | human | 283652 | SLC24A5 | solute carrier family 24 me... | XM_024449901.1 | 56.2% | 56.4% | 0_1ins339;843_845delTAA;849_849delCins316 |
| 3 | human | 283652 | SLC24A5 | solute carrier family 24 me... | XM_017022079.1 | 52.9% | 53.1% | (many diffs) |
| 4 | human | 283652 | SLC24A5 | solute carrier family 24 me... | XM_017022080.1 | 52.9% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1569
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagacaaaa gggggccaaa catgggcgag aagggctctg ttgctcggca 121 tcctgtgggc cactgcacat ctgcctctct cagggacctc cctgccccaa cgtctcccaa 181 gggccacagg aaatagcacc caatgtgtta tttctccatc atcggagttt cccgaagggt 241 ttttcacgag acaggagcgc agagatggag gcatcataat ctatttccta attatcgttt 301 acatgttcat ggccatatct attgtctgtg atgaatactt cctaccctcc ctggaaatca 361 tcagtgaatc ccttggattg tctcaggatg ttgcaggcac aactttcatg gcagcgggca 421 gttcagctcc tgaattagtt actgctttcc taggtgtatt tatcacaaag ggagatattg 481 gcattagcac catccttgga tctgcaattt ataatctcct tggcatctgt gctgcctgtg 541 gtttgctatc taatacggtc tcaacactat catgttggcc cctattcaga gactgtgcag 601 cgtacacaat tagtgcagca gcagttcttg gtataatata tgacaaccaa gtttactggt 661 atgaaggggc tttactgctt ttgatatatg gattgtatgt tttggtgctg tgttttgaca 721 ttaaaattaa ccaatatatt ataaagaaat gcagtccttg ctgcgcctgt cttgccaaag 781 ctatggagag aagtgaacaa cagccactga tgggctggga agatgaaggt caaccattca 841 ttcgtcggca atcaagaact gatagtggaa tattttatga agattctggc tactctcagc 901 tctctataag tttacatggc cttagtcagg tttctgaaga tccaccaagt gttttcaaca 961 tgcctgaagc agacttaaaa agaatttttt gggtattatc ccttcctatt attacattac 1021 tttttctaac cacaccagat tgtagaaaaa agttttggaa aaactacttt gtgataacct 1081 ttttcatgtc tgcaatatgg atatccgcat ttacatatat cctggtttgg atggtcacaa 1141 taactgggga aacactagaa attcccgata cagtaatggg ccTTACTTTA TTAGCAGCAG 1201 GAACAAGCAT ACCAGACACA ATTGCAAGTG TGTTGGTTGC AAGAAAAGGG AAAGGAGATA 1261 TGGCTATGTC TAACATCGTG GGATCCAATG TGTTTGATAT GTTGTGCCTT GGTATTCCAT 1321 GGTTTATTAA AACTGCATTT ATAAATGGAT CAGCTCCTGC AGAAGTAAAC AGCAGAGGAC 1381 TAACTTACAT AACCATCTCT CTCAACATTT CAATTATTTT TCTTTTTTTA GCAGTTCACT 1441 TCAATGGCTG GAAACTAGAC AGAAAGTTGG GAATAGTCTG CCTATTATCA TACTTGGGGC 1501 TTGCTACATT ATCAGTTCTA TATGAACTTG GAATTATTGG AAATAATAAA ATAAGGGGCT 1561 GTGGAGGTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGGCAG ACGGTATGTA AAAATCTAAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt