Construct: ORF TRCN0000466173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009451.1_s317c1
- Derived from:
- ccsbBroadEn_02235
- DNA Barcode:
- AGTTAAATACTTTACTAGTAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STARD8 (9754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9754 | STARD8 | StAR related lipid transfer... | NM_001142504.3 | 100% | 100% | |
| 2 | human | 9754 | STARD8 | StAR related lipid transfer... | NM_014725.4 | 100% | 100% | |
| 3 | human | 9754 | STARD8 | StAR related lipid transfer... | XM_011531069.3 | 100% | 100% | |
| 4 | human | 9754 | STARD8 | StAR related lipid transfer... | XM_005262315.3 | 99.6% | 99.6% | 55_66del |
| 5 | human | 9754 | STARD8 | StAR related lipid transfer... | XM_011531070.2 | 99.6% | 99.6% | 55_66del |
| 6 | human | 9754 | STARD8 | StAR related lipid transfer... | NM_001142503.2 | 92.7% | 92.7% | 1_240del |
| 7 | human | 9754 | STARD8 | StAR related lipid transfer... | XM_005262314.4 | 92.4% | 92.4% | 1_240del;295_306del |
| 8 | mouse | 236920 | Stard8 | START domain containing 8 | NM_199018.2 | 83.2% | 84.9% | (many diffs) |
| 9 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528004.2 | 83.2% | 84.9% | (many diffs) |
| 10 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528007.1 | 83.2% | 84.9% | (many diffs) |
| 11 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528005.1 | 81.9% | 83.2% | (many diffs) |
| 12 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528006.1 | 81.9% | 83.2% | (many diffs) |
| 13 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528003.3 | 81.6% | 82.2% | (many diffs) |
| 14 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006528002.1 | 81.5% | 82.1% | (many diffs) |
| 15 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006527999.3 | 77.5% | 79.1% | (many diffs) |
| 16 | mouse | 236920 | Stard8 | START domain containing 8 | XM_006527998.1 | 77.3% | 78.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3135
- ORF length:
- 3069
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cttgaataat tgtgcctcga tgaaactgga ggttcatttt caaagcaagc 121 agaatgaaga ctcagaagag gaagagcagt gtaccatcag tagccactgg gccttccagc 181 aggaaagtaa gtgctggtct cctatggggt cctctgatct gttggcccca ccgagccctg 241 gcctgccagc gacctcaagc tgtgagagcg tcctcaccga gcttagtgcc acctctctgc 301 cagtcatcac cgtgagccta ccacccgagc cagcagactt gcccttgcca ggccgtgccc 361 ccagctcgag tgaccggccc ctcctcagcc ccacccaggg ccaggagggt ccccaggaca 421 aagccaagaa gcgccatcgt aaccgtagct tcctcaagca ccttgaatct ctgaggcgga 481 aggaaaagag tggcagccag caagcagagc ccaagcatag tccagccacc tcagagaagg 541 tctccaaagc ctcatctttc cgcagttgtc gtggcttcct ctcagctgga ttttacaggg 601 ccaagaactg ggccgccacc tcagccggtg gcagtggtgc caatactcgg aaggcctggg 661 aggcctggcc tgtggcctcg ttccggcatc ctcagtggac acaccggggt gattgcctgg 721 tgcacgttcc tggggaccac aaaccaggca cattccctcg ctccctgtcc attgagagcc 781 tgtgtcctga ggatggacac cgcctggcag actggcagcc aggtaggcgg tggggctgtg 841 aggggcgccg gggctcctgt ggctcaacgg gcagccatgc cagcacgtat gacaacttgc 901 ctgagctgta cccagctgag cctgtaatgg ttggggctga ggctgaagat gaagatgatg 961 aggagagtgg gggcagctat gctcacctag acgacatcct ccagcacgtg tgggggctac 1021 agcaacgagt agagctgtgg tctcgggcca tgtacccaga cctggggcct ggagatgagg 1081 aagaggagga ggccacttca tcagtagaaa tagccacagt tgaggtcaaa tgccaagctg 1141 aggctctcag ccagatggag gttccggccc atggagagtc cccagcctgg gcccaggctg 1201 aagtccagcc agcagtcctg gctccggctc aggctccagc tgaggctgaa ccagtggcac 1261 aggaagaggc tgaggccccg gccccagccc cggccccggc cccagcccag gacagtgagc 1321 aggaggcaca ttcaggcggg gaacccacct ttgcctctag cctgtctgtg gaagaaggac 1381 actccatttc tgacactgtg gcctcctcca gcgaacttga cagtagtggg aactccatga 1441 atgaggctga ggctgcgggg cccctggctg gactccaggc atcaatgccc cgtgaacggc 1501 gcgattcagg tgttggggcc tcacttacca gaccctgcag gaagctccgt tggcatagct 1561 tccagaactc ccatcgtccc agcctcaact cagagtcgct ggagatcaac cggcagtttg 1621 caggccagat caacctcctg cacaagggct cactgctgcg gcttaccgcg ttcatggaga 1681 agtacactgt gccccacaag cagggctggg tctggtcaat gcccaagttc atgaggagga 1741 acaagacccc agattaccgg ggacagcacg tatttggggt gccacccctc atccacgtgc 1801 agcgcacggg ccagccactg ccacagagca ttcagcaagc catgcgctac ttgcgcagcc 1861 agtgcctgga ccaagtaggc atcttccgca agtctggggt caagtccagg atccagaacc 1921 tgcgtcaaat gaatgagacc tcgcctgaca atgtctgcta cgagggccag tcagcctacg 1981 acgtggctga cctgctaaag cagtatttcc gggacctgcc tgagcccatc ttcaccagca 2041 agctcaccac cactttcctc cagatctacc agctcctccc caaggatcag tggttggcag 2101 cagcacaagc cgccaccttg ctgctccccg atgagaaccg agaggtgcta cagaccctgc 2161 tctacttctt aagtgacatt gcctctgccg aggaaaacca gatgacagca ggcaacctgg 2221 cagtgtgcct ggcgccctcc atcttccacc tcaatgtctc taagaaggat agcccctctc 2281 ccaggatcaa gagcaaacgc agcctcattg gcaggccagg ccctagggac ctgagtgaca 2341 acatggcagc cacccagggc ctgtcgcaca tgatcagtga ctgcaagaaa cttttccagg 2401 tgccccagga catggtgctg cagctgtgca gctcctacag cgcagctgag ctcagccctc 2461 ccggcccagc cctggctgag ctgcgtcagg cccaagctgc aggggtaagc ctgagcctct 2521 acatggaaga gaatatccag gacctgctgc gtgatgctgc tgagcgcttc aagggctgga 2581 tgagcgtgcc agggccccag cacacggagc tggcttgcag gaaggcaccg gatgggcacc 2641 ccctgcggct atggaaggca tccacagagg tggcagcccc cccagctgtg gtgctgcatc 2701 gtgttctccg ggagcgggcc ctctgggatg aggatctgct gcgggcccag gtgctggaag 2761 ccctgatgcc gggtgtggag ctgtacCACT ATGTCACCGA CAGCATGGCA CCCCATCCCT 2821 GCCGCGACTT TGTGGTGCTT CGGATGTGGC GCTCTGACCT GCCTCGTGGG GGTTGCCTGC 2881 TTGTCTCCCA GTCCCTGGAT CCGGAACAAC CTGTGCCAGA GTCGGGTGTG CGAGCCCTCA 2941 TGCTCACATC CCAGTACCTC ATGGAGCCTT GCGGCTTGGG CCGCTCTCGG CTCACACACA 3001 TCTGCCGGGC TGACCTCAGG GGCCGTTCTC CTGACTGGTA CAACAAAGTC TTTGGACACC 3061 TGTGTGCCAT GGAAGTGGCA AAGATCCGGG ACTCCTTCCC CACCCTGCAG GCAGCGGGCC 3121 CTGAGACAAA GCTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3181 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3241 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGTTAAATAC TTTACTAGTA 3301 GTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt