Construct: ORF TRCN0000466191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013199.1_s317c1
- Derived from:
- ccsbBroadEn_02139
- DNA Barcode:
- GATACAAGTAGCGGCCGGCTATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN3 (9324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466191
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_138730.3 | 100% | 100% | |
| 2 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001201362.2 | 80% | 81% | (many diffs) |
| 3 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_004242.4 | 77.7% | 77.7% | 219_259del;273_297del |
| 4 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318888.2 | 69.8% | 63.6% | (many diffs) |
| 5 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318884.2 | 61.2% | 62% | (many diffs) |
| 6 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318885.2 | 60.6% | 60.6% | 13_14ins51;168_208del;222_246del |
| 7 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318886.2 | 59.3% | 60.1% | (many diffs) |
| 8 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001201363.2 | 58.4% | 59.2% | (many diffs) |
| 9 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318887.2 | 58.4% | 59.2% | (many diffs) |
| 10 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | NM_175074.2 | 91.3% | 92.2% | (many diffs) |
| 11 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | NM_026122.4 | 71% | 69.6% | (many diffs) |
| 12 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | XM_006511590.3 | 53.4% | 52.6% | (many diffs) |
| 13 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | XM_006511591.3 | 53.4% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 373
- ORF length:
- 231
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGCCGAAG AGAAAGTCTC CAGAGAATAC AGAGGGCAAA 181 GATGGATCCA AAGTAACTAA ACAGGAGCCC ACAAGACGGT CTGCCAGATT GTCAGCGAAA 241 CCTGCTCCAC CAAAACCTGA ACCCAAACCA AGAAAAACAT CTGCTAAGAA AGAACCTGGA 301 GCAAAGATTA GCAGAGGTGC TAAAGGGAAG AAGGAGGAAA AGCAGGAAGC TGGAAAGGAA 361 GGCACAGAAA ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 421 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 481 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA TACAAGTAGC GGCCGGCTAT 541 GCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt