Construct: ORF TRCN0000466193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017049.1_s317c1
- Derived from:
- ccsbBroadEn_04318
- DNA Barcode:
- TTTCGGACACACCTGAGTGTAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MND1 (84057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466193
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84057 | MND1 | meiotic nuclear divisions 1 | NM_032117.4 | 100% | 100% | |
| 2 | human | 84057 | MND1 | meiotic nuclear divisions 1 | XM_017008688.2 | 92.6% | 92.6% | 0_1ins45 |
| 3 | human | 84057 | MND1 | meiotic nuclear divisions 1 | XM_005263275.2 | 92.6% | 92.1% | 466_467ins45 |
| 4 | human | 84057 | MND1 | meiotic nuclear divisions 1 | XM_024454242.1 | 85.3% | 84.8% | 0_1ins45;421_422ins45 |
| 5 | human | 84057 | MND1 | meiotic nuclear divisions 1 | NM_001253861.1 | 59% | 57% | (many diffs) |
| 6 | human | 84057 | MND1 | meiotic nuclear divisions 1 | NR_045605.2 | 56.4% | 1_50del;325_485del;827_1090del | |
| 7 | mouse | 76915 | Mnd1 | meiotic nuclear divisions 1 | NM_029797.3 | 86% | 89.7% | (many diffs) |
| 8 | mouse | 76915 | Mnd1 | meiotic nuclear divisions 1 | XM_006502262.3 | 76.4% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aaagaaaaaa ggactgagtg cagaagaaaa gagaactcgc atgatggaaa 121 tattttctga aacaaaagat gtatttcaat taaaagactt ggagaagatt gctcccaaag 181 agaaaggcat tactgctatg tcagtaaaag aagtccttca aagcttagtt gatgatggta 241 tggttgactg tgagaggatc ggaacttcta attattattg ggcttttcca agtaaagctc 301 ttcatgcaag gaaacataag ttggaggttc tggaatctca gttGTCTGAG GGAAGTCAAA 361 AGCATGCAAG CCTACAGAAA AGCATTGAGA AAGCTAAAAT TGGCCGATGT GAAACGGAAG 421 AGCGAACCAG GCTAGCAAAA GAGCTTTCTT CACTTCGAGA CCAAAGGGAA CAGCTAAAGG 481 CAGAAGTAGA AAAATACAAA GACTGTGATC CGCAAGTTGT GGAAGAAATA CGCCAAGCAA 541 ATAAAGTAGC CAAAGAAGCT GCTAACAGAT GGACTGATAA CATATTCGCA ATAAAATCTT 601 GGGCCAAAAG AAAATTTGGG TTTGAAGAAA ATAAAATTGA TAGAACTTTT GGAATTCCAG 661 AAGACTTTGA CTACATAGAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTC GGACACACCT 841 GAGTGTAAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt