Construct: ORF TRCN0000466197

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005877.2_s317c1
Derived from:
ccsbBroadEn_01918
DNA Barcode:
GTTTCGTTATTGTGGGATGTGTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NME5 (8382)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466197
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8382 NME5 NME/NM23 family member 5 NM_003551.3 100% 100%
2 human 8382 NME5 NME/NM23 family member 5 XM_017009945.2 72.4% 67.9% (many diffs)
3 human 8382 NME5 NME/NM23 family member 5 XM_024446227.1 72.4% 67.9% (many diffs)
4 human 8382 NME5 NME/NM23 family member 5 XM_024446228.1 69.8% 68.3% (many diffs)
5 human 8382 NME5 NME/NM23 family member 5 XM_005272099.2 69.4% 68.3% 437A>T;440_440delAinsTT;444_445ins191
6 mouse 75533 Nme5 NME/NM23 family member 5 NM_080637.3 83.3% 85.8% (many diffs)
7 mouse 75533 Nme5 NME/NM23 family member 5 XM_017318004.1 83.3% 85.8% (many diffs)
8 mouse 75533 Nme5 NME/NM23 family member 5 XM_006526319.2 75.1% 72.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
702
ORF length:
636
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gatatcaatg cctccacctc agatatatgt agaaaaaact ctggccatta
121tcaaaccaga tattgttgac aaagaggagg agatacaaga tattattctt agatccggat
181tcaccattgt tcagagaaga aaactacgcc tcagccctga gcaatgtagt aacttttatg
241tggaaaagta tggaaaaatg tttttcccca acttaacagc ttacatgagt tctggaccac
301ttgtcgccat gatattagct agacataaag ccatctctta ttggttagaa cttttgggac
361caaataatag cttagtagcg aaggagacac atCCAGACAG TCTGAGGGCA ATTTATGGCA
421CAGATGACCT AAGGAATGCA CTTCATGGGA GTAATGACTT TGCTGCTGCG GAAAGAGAAA
481TACGTTTTAT GTTTCCTGAA GTGATTGTTG AGCCCATTCC AATTGGACAA GCTGCTAAGG
541ACTATTTAAA TTTACATATA ATGCCAACTC TGCTTGAAGG ACTCACAGAG CTTTGTAAGC
601AAAAACCAGC AGATCCTTTG ATTTGGCTAG CTGATTGGCT GCTGAAAAAT AATCCTAACA
661AACCCAAACT TTGTCACCAT CCAATTGTAG AAGAACCTTA TTACCCAACT TTCTTGTACA
721AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
781AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
841AGGACGAGTT TCGTTATTGT GGGATGTGTT CACGCGTTAA GTCgacaatc aacctctgga
901ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)