Construct: ORF TRCN0000466219

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011031.1_s317c1
Derived from:
ccsbBroadEn_11972
DNA Barcode:
GCATTGATCAACGAAACTATTGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GASK1B (51313)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466219
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51313 GASK1B golgi associated kinase 1B NM_001128424.2 47.3% 47.3% 1_819del
2 human 51313 GASK1B golgi associated kinase 1B NM_016613.7 47.3% 47.3% 1_819del
3 human 51313 GASK1B golgi associated kinase 1B XM_024454078.1 47.3% 47.3% 1_819del
4 human 51313 GASK1B golgi associated kinase 1B XM_024454079.1 47.3% 47.3% 1_819del
5 human 51313 GASK1B golgi associated kinase 1B NM_001031700.3 44.4% 42.1% (many diffs)
6 mouse 68659 Fam198b family with sequence simila... NM_133187.3 41.1% 42.3% (many diffs)
7 mouse 68659 Fam198b family with sequence simila... XM_006501984.3 41.1% 42.3% (many diffs)
8 mouse 68659 Fam198b family with sequence simila... XM_006501985.2 41.1% 42.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
804
ORF length:
738
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag tgaggtgttt gccttccacc tagacaggat cctggggctc aacaggaccc
121tgccgtctgt gagcaggaaa gcagagttca tccaagatgg ccgcccatgc cccatcattc
181tttgggatgc atctttatct tcagcaagta atgacaccca ttcttctgtt aagctcacct
241ggggaactta tcagcagttg ctgaaacaga aatgctggca gaatggccga gtacccaagc
301ctgaatcggg ttgtactgaa atacatcatc atgagtggTC CAAGATGGCA CTCTTTGATT
361TTTTGTTACA GATTTATAAT CGCTTAGATA CAAATTGCTG TGGATTCAGA CCTCGCAAGG
421AAGATGCCTG TGTACAGAAT GGATTGAGGC CAAAATGTGA TGACCAAGGT TCTGCGGCTC
481TAGCACACAT TATCCAGCGA AAGCATGACC CAAGGCATTT GGTTTTTATA GACAACAAGG
541GTTTCTTTGA CAGGAGTGAA GATAACTTAA ACTTCAAATT GTTAGAAGGC ATCAAAGAGT
601TTCCAGCTTC TGCAGTTTCT GTTTTGAAGA GCCAGCACTT ACGGCAGAAA CTTCTTCAGT
661CTCTGTTTCT TGATAAAGTG TATTGGGAAA GTCAAGGAGG TAGACAAGGA ATTGAAAAGC
721TTATCGATGT AATAGAACAC AGAGCCAAAA TTCTTATCAC CTATATCAAT GCACACGGGG
781TCAAAGTATT ACCTATGAAT GAATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
841AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
901TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CATTGATCAA
961CGAAACTATT GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1021att

Download FASTA (ORF) (Full)