Construct: ORF TRCN0000466219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011031.1_s317c1
- Derived from:
- ccsbBroadEn_11972
- DNA Barcode:
- GCATTGATCAACGAAACTATTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GASK1B (51313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51313 | GASK1B | golgi associated kinase 1B | NM_001128424.2 | 47.3% | 47.3% | 1_819del |
| 2 | human | 51313 | GASK1B | golgi associated kinase 1B | NM_016613.7 | 47.3% | 47.3% | 1_819del |
| 3 | human | 51313 | GASK1B | golgi associated kinase 1B | XM_024454078.1 | 47.3% | 47.3% | 1_819del |
| 4 | human | 51313 | GASK1B | golgi associated kinase 1B | XM_024454079.1 | 47.3% | 47.3% | 1_819del |
| 5 | human | 51313 | GASK1B | golgi associated kinase 1B | NM_001031700.3 | 44.4% | 42.1% | (many diffs) |
| 6 | mouse | 68659 | Fam198b | family with sequence simila... | NM_133187.3 | 41.1% | 42.3% | (many diffs) |
| 7 | mouse | 68659 | Fam198b | family with sequence simila... | XM_006501984.3 | 41.1% | 42.3% | (many diffs) |
| 8 | mouse | 68659 | Fam198b | family with sequence simila... | XM_006501985.2 | 41.1% | 42.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaggtgttt gccttccacc tagacaggat cctggggctc aacaggaccc 121 tgccgtctgt gagcaggaaa gcagagttca tccaagatgg ccgcccatgc cccatcattc 181 tttgggatgc atctttatct tcagcaagta atgacaccca ttcttctgtt aagctcacct 241 ggggaactta tcagcagttg ctgaaacaga aatgctggca gaatggccga gtacccaagc 301 ctgaatcggg ttgtactgaa atacatcatc atgagtggTC CAAGATGGCA CTCTTTGATT 361 TTTTGTTACA GATTTATAAT CGCTTAGATA CAAATTGCTG TGGATTCAGA CCTCGCAAGG 421 AAGATGCCTG TGTACAGAAT GGATTGAGGC CAAAATGTGA TGACCAAGGT TCTGCGGCTC 481 TAGCACACAT TATCCAGCGA AAGCATGACC CAAGGCATTT GGTTTTTATA GACAACAAGG 541 GTTTCTTTGA CAGGAGTGAA GATAACTTAA ACTTCAAATT GTTAGAAGGC ATCAAAGAGT 601 TTCCAGCTTC TGCAGTTTCT GTTTTGAAGA GCCAGCACTT ACGGCAGAAA CTTCTTCAGT 661 CTCTGTTTCT TGATAAAGTG TATTGGGAAA GTCAAGGAGG TAGACAAGGA ATTGAAAAGC 721 TTATCGATGT AATAGAACAC AGAGCCAAAA TTCTTATCAC CTATATCAAT GCACACGGGG 781 TCAAAGTATT ACCTATGAAT GAATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CATTGATCAA 961 CGAAACTATT GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att