Construct: ORF TRCN0000466228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014532.1_s317c1
- Derived from:
- ccsbBroadEn_06416
- DNA Barcode:
- AGCATGCCTTTTACGACAAGATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR1D (3352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3352 | HTR1D | 5-hydroxytryptamine recepto... | NM_000864.4 | 99.9% | 100% | 585C>T |
| 2 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285482.1 | 85% | 90.1% | (many diffs) |
| 3 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285483.1 | 85% | 90.1% | (many diffs) |
| 4 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285484.1 | 85% | 90.1% | (many diffs) |
| 5 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_008309.5 | 85% | 90.1% | (many diffs) |
| 6 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538577.3 | 85% | 90.1% | (many diffs) |
| 7 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538578.2 | 85% | 90.1% | (many diffs) |
| 8 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_017319986.2 | 85% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cccactgaac cagtcagcag aaggccttcc ccaggaggcc tccaacagat 121 ccctgaatgc cacagaaacc tcagaggctt gggatcccag gaccctccag gcgctcaaga 181 tctcccttgc cgtggtcctt tccgtcatca cactggccac agtcctctcc aatgcctttg 241 tactcaccac catcttactc accaggaagc tccacacccc tgccaactac ctgattggct 301 ccctggccac caccgacctc ttggtttcca tcttggtaat gcccatcagc atcgcctata 361 ccatcaccca cacctggaac tttggccaaa tcttgtgtga catctggctg tcctctgaca 421 tcacgtgctg cacagcctcc atcctgcatc tctgtgtcat tgctctggac aggtactggg 481 caatcacaga tgccctggaa tacagtaaac gcaggacggc tggccacgcg gccaccatga 541 tcgccattgt ctgggccatc tccatctgca tctccatccc cccgctcttc tggcggcagg 601 ccaaggccca ggaggagatg tcggactgtc tggtgaacac ctctcagatt tcctacacca 661 tctactccac ctgtggggcc ttctacattc cctcggtgtt gctcatcatc ctatatggcc 721 ggatctaccg ggctgcccgg aaccgcatcc tgaatccacc ctcactctat gggaagcgct 781 tcaccacggc ccacctcatc acaggctctg ccgggtcctc gctctgctcg ctcaactcca 841 gcctccatga ggggcactcg cactcggctg gctcccctct ctttttcaac cacgtgaaaa 901 tcaagcttgc tgacagtgcc ctggaacgca agaggatttc tgctgctcga gaaaggaaag 961 ccactaaaat cctgggcatc attctggggg cctttatcat ctgctggctg cccttcttcg 1021 tggtgtctct ggtcctcccc atctgccggg actcctgctg gatccacccg gcgctctttg 1081 acttcttcac ctGGCTAGGC TATTTAAACT CCCTCATCAA TCCAATAATC TACACTGTGT 1141 TTAATGAAGA GTTTCGGCAA GCTTTTCAGA AAATTGTCCC TTTCCGGAAG GCCTCCTACC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAAGCATGCC TTTTACGACA AGATGGACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt