Construct: ORF TRCN0000466231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003793.1_s317c1
- Derived from:
- ccsbBroadEn_04258
- DNA Barcode:
- GACTTACACAGACTGATTATAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST6GALNAC5 (81849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466231
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81849 | ST6GALNAC5 | ST6 N-acetylgalactosaminide... | NM_030965.3 | 100% | 100% | |
| 2 | human | 81849 | ST6GALNAC5 | ST6 N-acetylgalactosaminide... | NM_001320273.2 | 32.1% | 27.1% | 259_260ins410;324_325ins274 |
| 3 | human | 81849 | ST6GALNAC5 | ST6 N-acetylgalactosaminide... | NM_001320274.2 | 27.7% | 26.1% | (many diffs) |
| 4 | human | 81849 | ST6GALNAC5 | ST6 N-acetylgalactosaminide... | XR_001737460.2 | 15% | 1_227del;1236_6711del | |
| 5 | mouse | 26938 | St6galnac5 | ST6 (alpha-N-acetyl-neurami... | NM_012028.4 | 88.7% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaccctgatg cgccatggtc tggcagtgtg tttagcgctc accaccatgt 121 gcaccagctt gttgctagtg tacagcagcc tcggcggcca gaaggagcgg cccccgcagc 181 agcagcagca gcagcagcaa cagcagcagc aggcgtcggc caccggcagc tcgcagccgg 241 cggcggagag cagcacccag cagcgccccg gggtccccgc gggaccgcgg ccactggacg 301 gatacctcgg agtggcggac cacaagcccc tgaaaatgca ctgcagggac tgtgccctgg 361 tgaccagctc agggcatctg ctgcacagtc ggcaaggctc ccagattgac cagacagagt 421 gtgtcatccg catgaatgac gcccccacac gcggctatgg gcgtgacgtg ggcaatcgca 481 ccagcctgag ggtcatcgcg cattccagca tccagaggat cctccgcaac cgccatgacc 541 tgctcaacgt gagccagggc accgtgttca tcttctgggg ccccagcagc tacatgcggc 601 gggacggcaa gggccaggtc tacaacaacc tgcatctcct gagccaggtg ctgccccggc 661 tgaaggCCTT CATGATTACT CGCCACAAGA TGCTGCAGTT TGATGAGCTC TTCAAGCAGG 721 AGACTGGCAA AGACAGGAAG ATATCCAACA CTTGGCTCAG CACTGGCTGG TTTACAATGA 781 CAATTGCACT GGAGCTCTGT GACAGGATCA ATGTTTATGG CATGGTGCCC CCAGACTTCT 841 GCAGGGATCC CAATCACCCT TCAGTACCTT ATCATTATTA TGAACCTTTT GGACCTGATG 901 AATGTACAAT GTACCTCTCC CATGAGCGAG GACGCAAGGG CAGTCATCAC CGCTTTATCA 961 CAGAGAAACG AGTCTTTAAG AACTGGGCAC GGACATTCAA TATTCACTTT TTTCAACCAG 1021 ACTGGAAACC AGAATCACTT GCTATAAATC ATCCTGAGAA TAAACCTGTG TTCTACCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAG ACTTACACAG ACTGATTATA ATAACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att