Construct: ORF TRCN0000466251
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002719.1_s317c1
- Derived from:
- ccsbBroadEn_03065
- DNA Barcode:
- ACCCCGAATGCTCAGGTGATCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf72 (29035)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466251
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29035 | C16orf72 | chromosome 16 open reading ... | NM_014117.3 | 100% | 100% | |
| 2 | human | 29035 | C16orf72 | chromosome 16 open reading ... | XM_011522462.3 | 79% | 70.2% | (many diffs) |
| 3 | mouse | 69053 | 1810013L24Rik | RIKEN cDNA 1810013L24 gene | NM_001081400.3 | 97% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 894
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggagcgg aaggaggagg gcgaggccga gatccaggag cacgggcccg 121 agcactggtt ctccaagtgg gagcggcagt gcctggccga ggccgaacag gacgagcagc 181 tgccccccga gctgcaggag gaggcggcgg ccgccgcgca gcccgagcac aagcagcaga 241 agctgtggca cctcttccag aactcggcca ccgccgtggc ccagctctac aaagaccgag 301 tgtgtcagca gccaggactt tctctctggg tccccttcca aaacgcagcc accgccgtca 361 ccaatctcta caaagaaagc gtggataccc atcaacgaag ttttgatatt ggaattcaga 421 ttggctatca gcgacgcaat aaggatgtgt tggcttgggt taaaaaacgc agaagaacta 481 ttcgtcgaga agatttgatc agcttcctgt gtggaaaagt tcctccacca cgaaactcta 541 gagctccccC AAGACTGACT GTAGTGTCCC CTAACCGAGC TACTTCAACG GAAACTAGCT 601 CATCTGTAGA GACTGATTTG CAACCCTTCC GGGAAGCCAT AGCTCTGCAT GGTCTTAGTG 661 GTGCAATGGC TAGTATAAGC GTGCGTTCGA GTACCCCAGG CTCTCCTACA CATGTAAGCA 721 GTGGATCGAA TGCTAGTCGA AGGAGAAATG GACTCCATGA TGTCGATTTG AACACTTTCA 781 TATCAGAAGA AATGGCACTC CACTTGGACA ATGGTGGAAC TAGAAAGCGT ACCTCAGCCC 841 AGTGTGGCGA TGTCATTACA GACTCACCAA CCCATAAACG CAACAGAATG ATCTTGCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1021 ATCTTGTGGA AAGGACGAAC CCCGAATGCT CAGGTGATCT TAACGCGTTA AGTCgacaat 1081 caacctctgg attacaaaat ttgtgaaaga tt