Construct: ORF TRCN0000466255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004005.1_s317c1
- Derived from:
- BRDN0000395116
- DNA Barcode:
- GTGGTTTGCAATCGTTCGATCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF521 (25925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25925 | ZNF521 | zinc finger protein 521 | NM_015461.3 | 99.8% | 99.8% | 20C>A;3225_3227delTTCinsCCG;3597G>A |
| 2 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_011525909.3 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_011525910.3 | 92.2% | 90.6% | (many diffs) |
| 4 | human | 25925 | ZNF521 | zinc finger protein 521 | NM_001308225.2 | 83.1% | 83.1% | 0_1ins660;2565_2567delTTCinsCCG;2937G>A |
| 5 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_011525911.2 | 83.1% | 83.1% | 0_1ins660;2565_2567delTTCinsCCG;2937G>A |
| 6 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_017025698.2 | 83.1% | 83.1% | 0_1ins660;2565_2567delTTCinsCCG;2937G>A |
| 7 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_024451141.1 | 83.1% | 83.1% | 0_1ins660;2565_2567delTTCinsCCG;2937G>A |
| 8 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_024451142.1 | 83.1% | 83.1% | 0_1ins660;2565_2567delTTCinsCCG;2937G>A |
| 9 | human | 25925 | ZNF521 | zinc finger protein 521 | XM_017025697.2 | 83% | 83% | (many diffs) |
| 10 | mouse | 225207 | Zfp521 | zinc finger protein 521 | NM_145492.4 | 88.1% | 97.3% | (many diffs) |
| 11 | mouse | 225207 | Zfp521 | zinc finger protein 521 | XM_011246894.1 | 88% | 97.2% | (many diffs) |
| 12 | mouse | 225207 | Zfp521 | zinc finger protein 521 | XM_006525819.3 | 73.4% | 81.1% | (many diffs) |
| 13 | mouse | 225207 | Zfp521 | zinc finger protein 521 | XM_006525820.3 | 73.4% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4002
- ORF length:
- 3933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctcgccgc aagcaagaga aaccgagatc cctcaaagac cccaactgta 121 aacttgaaga caagactgaa gatggagagg cactagattg taagaagagg ccggaagacg 181 gggaggagtt ggaagacgaa gctgtgcaca gctgtgacag ctgcctccag gtgtttgaat 241 cgctgagcga tatcacagaa cacaagatta atcaatgtca actgacagat ggagtggatg 301 ttgaagatga tccgacttgc tcttggccag cttcctcacc ttctagcaag gatcagactt 361 cccctagcca tggagaaggt tgcgattttg gagaggaaga aggtggccct gggcttccat 421 acccgtgtca attctgtgac aagtcgttta gccgcctcag ctacctaaag caccatgagc 481 agagtcacag tgacaaactg cctttcaaat gcacctactg cagtaggctg ttcaaacaca 541 agcgcagccg agatcgccac ataaaactcc acaccgggga caagaagtac cactgcagtg 601 aatgtgatgc tgcgttttcc agaagtgatc acttgaagat ccacttaaag actcacacgt 661 ccaacaagcc atataaatgt gccatttgtc gccgtgggtt tctgtcctct agttccttac 721 acggacacat gcaggttcat gagaggaaca aggacggctc tcagtccggt tccaggatgg 781 aggactggaa gatgaaggac actcagaagt gcagtcagtg tgaggaaggc tttgacttcc 841 cggaagacct ccaaaaacac attgcagagt gccaccccga atgctcccca aatgaggacc 901 gagcggccct ccagtgtgtc tactgccacg agctcttcgt agaggagacc tccctcatga 961 accacatgga gcaggtgcat agcggggaga agaagaactc atgcagcatt tgttctgaga 1021 gtttccacac agttgaggaa ctgtacagcc acatggacag tcaccagcaa ccggagtcat 1081 gcaatcacag caacagccct tccctggtca cggtgggcta tacctccgtg tccagtacga 1141 ctccagattc caacctctca gtggacagct caaccatggt ggaagctgcc ccgccaatcc 1201 caaagagtcg agggaggaag agggccgctc aacaaacccc tgacatgact ggtccctcga 1261 gtaaacaagc aaaagttacc tacagctgta tttactgcaa caaacaatta ttttcaagtc 1321 ttgcagttct gcagattcac ctgaaaacta tgcacttaga taagccagaa caggcccata 1381 tttgtcagta ttgcttggag gtcctgccct cactctataa cctaaatgaa catcttaagc 1441 aagtgcatga agctcaggac ccaggtctga ttgtttctgc catgcctgcc attgtctacc 1501 agtgtaactt ctgttccgaa gttgtcaacg acctcaacac tcttcaggaa cacatccgat 1561 gttctcatgg atttgcaaac cctgcagcta aagatagtaa tgcattcttt tgtccccatt 1621 gctatatggg gtttctcact gactcttccc tggaagagca tattagacag gttcattgtg 1681 acctcagtgg ctcccgattt gggtctccag tgcttgggac tcccaaagaa ccagtagtag 1741 aagtctattc ttgttcctat tgtacaaatt cgccaatatt caacagcgtt cttaaactga 1801 acaagcatat caaagagaat cataaaaaca ttcccttggc cctgaattat atccacaatg 1861 ggaagaaatc cagggcctta agccccctat ctcctgtggc catagagcag acatctctta 1921 agatgatgca ggcagtagga ggtgcacctg cacgtcccac tggagaatat atctgtaatc 1981 aatgtggtgc taagtacaca tccctagaca gctttcagac tcacctaaaa actcatctcg 2041 acactgtgct tccaaaattg acctgtcctc agtgcaacaa ggaattcccc aaccaagaat 2101 ccttgctgaa gcatgttacc attcacttta tgatcacttc aacgtattac atctgtgaga 2161 gttgtgacaa gcaattcaca tcagtggatg accttcagaa acacctgctg gacatgcaca 2221 cctttgtctt ctttcgctgc accctctgcc aggaagtttt tgactcaaaa gtctccattc 2281 agctccactt ggctgtgaag cacagtaacg aaaagaaagt ctataggtgc acatcttgca 2341 actgggactt ccgcaacgaa actgacttgc agctccatgt gaaacacaac cacctggaaa 2401 accaagggaa agtgcataag tgcattttct gcggtgagtc ctttggcacc gaggtggagc 2461 tgcaatgcca catcaccact cacagtaaga agtacaactg caagttctgt agcaaagcct 2521 tccatgcgat cattttgtta gaaaaacact tgcgagaaaa acactgtgta ttcgaaacca 2581 agacacccaa ctgtggaaca aatggagctt ccgagcaagt gcagaaagag gaagtggagc 2641 tgcagacttt gctgaccaac agccaggagt cccacaacag tcacgatggg agcgaagaag 2701 acgttgacac ctctgagcct atgtacggct gcgacatttg tggggcagcc tacactatgg 2761 aaactttgct gcagaatcac cagctccgag accacaacat cagacctgga gaaagtgcca 2821 tcgtgaaaaa gaaagctgag ctcattaaag ggaattacaa gtgcaacgtg tgctctcgaa 2881 ccttcttctc cgaaaatggc ctccgggaac atatgcagac ccacctaggc cctgtcaaac 2941 actacatgtg ccctatttgc ggagagcggt ttccctccct tttaactctt actgaacaca 3001 aagtcacgca tagtaagagt cttgatactg gaaactgccg gatttgcaag atgcctctcc 3061 agagtgaaga ggagttttta gagcattgcc aaatgcaccc tgacttgagg aattccctga 3121 caggctttcg ctgcgtggtg tgcatgcaga cagtgacctc caccttggaa ctcaaaatcc 3181 atgggacgtt ccacatgcaa aagacaggga atgggtctgc agttcagacc acagggcggg 3241 gccagcacgt ccaaaaactg tataagtgcg catcttgcct caaagaattc cgccgcaagc 3301 aagatctggt gaaacttgat atcaatggcc tgccatatgg tctgtgtgcc ggctgcgtga 3361 atctcagtaa gagcgccagc ccaggcatta acgtccctcc cggcacgaat agaccaggct 3421 tgggccagaa tgagaatctg agtgccattg aggggaaagg caaggtgggg ggactgaaga 3481 cacgctgctc tagctgcaac gttaagtttg agtctgaaag tgaactccag aaccacatcc 3541 aaaccatcca ccgagagctc gtgccagaca gcaacagcac acagttgaaa acgccccaag 3601 tatcaccaat gcccagaatc agtcccTCCC AGTCGGATGA GAAGAAGACC TATCAATGCA 3661 TCAAATGTCA GATGGTTTTC TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA 3721 TGATTGATGA AGGACTGAAC CATGAATGCA AACTCTGCAG CCAGACCTTT GACTCTCCTG 3781 CCAAACTCCA GTGCCACCTG ATAGAGCACA GCTTCGAAGG GATGGGAGGC ACCTTCAAGT 3841 GTCCAGTCTG CTTTACAGTA TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG 3901 CCCATGGACA AGAAGACAAG ATCTATGACT GTACACAATG TCCACAGAAG TTTTTCTTCC 3961 AAACAGAGCT GCAGAATCAT ACAATGACCC AACACAGCAG TTTGCCAACT TTCTTGTACA 4021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 4081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 4141 AGGACGAGTG GTTTGCAATC GTTCGATCAT AACGCGTTAA GTCgacaatc aacctctgga 4201 ttacaaaatt tgtgaaagat t