Construct: ORF TRCN0000466257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012872.1_s317c1
- Derived from:
- ccsbBroadEn_00321
- DNA Barcode:
- AGAATTAAGGAGCTCTCTAGTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLCN4 (1183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1183 | CLCN4 | chloride voltage-gated chan... | NM_001830.4 | 100% | 100% | |
| 2 | human | 1183 | CLCN4 | chloride voltage-gated chan... | NM_001256944.1 | 87.6% | 87.6% | 0_1ins282 |
| 3 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | NM_011334.4 | 86.7% | 96.8% | (many diffs) |
| 4 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_006539501.3 | 86.7% | 96.8% | (many diffs) |
| 5 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_006539502.3 | 86.7% | 96.8% | (many diffs) |
| 6 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_011250426.2 | 86.7% | 96.8% | (many diffs) |
| 7 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_006539503.3 | 83.7% | 91.6% | (many diffs) |
| 8 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_006539504.3 | 83.3% | 93% | (many diffs) |
| 9 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | NM_001302387.1 | 83% | 92.8% | (many diffs) |
| 10 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_017321961.1 | 83% | 92.8% | (many diffs) |
| 11 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | NM_001302386.1 | 79.6% | 89% | (many diffs) |
| 12 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XM_006539507.3 | 77.1% | 86.3% | (many diffs) |
| 13 | mouse | 12727 | Clcn4 | chloride channel, voltage-s... | XR_391040.3 | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2349
- ORF length:
- 2280
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcaatgcg ggagcgatga gtggctctgg aaacctgatg gatttcctcg 121 atgagccgtt ccctgatgtg gggacgtatg aggacttcca caccatcgac tggctaaggg 181 aaaagtcacg ggacaccgac agacacagga agatcaccag caagagcaag gagtccatat 241 gggagttcat caagagcctg ctggatgcct ggtcgggatg ggtggtgatg ctgctcatcg 301 gcctgctggc gggcaccttg gctggggtca tcgatctcgc cgtggactgg atgacggacc 361 tgaaggaggg ggtctgcctg tctgccttct ggtatagcca tgagcagtgt tgctggactt 421 ctaacgagac cacttttgag gacagagaca agtgtcccct gtggcagaaa tggtcggagc 481 tgctggtgaa tcagtcagag ggtgccagtg cttacattct gaattactta atgtacatcc 541 tatgggcgct gctgtttgca tttttggctg tctccctggt gcgtgtattt gcaccatatg 601 cctgtggctc tggcatacca gagataaaga ccattttgag cggctttatc atcaggggct 661 acttggggaa gtggaccctg ctaatcaaga cagtcacgct ggtgctggta gtgtcctccg 721 gtctgagcct tgggaaggaa gggccgctag tgcacgtggc ttgttgctgt ggcaacttct 781 tcagcagcct tttctccaag tacagcaaga atgagggcaa gaggcgggag gtgctttcag 841 ctgcagcggc tgctggagtc tctgttgcct ttggtgcacc aattggaggc gtgcttttca 901 gtctagaaga ggtcagttac tactttcccc tgaagacctt gtggaggtca tttttcgcag 961 ccctggtggc ggcctttacg ctgagatcca tcaatccctt tgggaatagc cgtctcgttc 1021 tcttttatgt ggaataccac acgccctggt acatggctga actcttcccc ttcatcctgc 1081 ttggggtctt cgggggcttg tggggaaccc tcttcatccg ctgcaacatc gcctggtgca 1141 ggaggcgcaa gaccaccagg ctggggaagt acccggtgct ggaggtcatt gtggtgactg 1201 ccatcactgc catcattgcc taccccaatc cctacacacg ccagagcacc agcgagctca 1261 tttctgagct gttcaatgac tgtggagccc ttgagtcttc ccagctctgt gactacatca 1321 atgaccccaa catgactcgg cctgtggatg acattccaga ccggccggct ggtgtcggtg 1381 tttacacggc catgtggcag ctggccctgg cactgatctt caaaatcgtc gttaccatat 1441 ttacctttgg catgaagatc ccgtcgggcc tcttcatccc cagcatggct gtgggcgcga 1501 tagcgggcag gatggtggga attggcgtgg agcagctggc ctaccatcac catgactgga 1561 tcatcttcag gaactggtgc agacccggtg cagactgtgt cacgccaggg ctgtacgcaa 1621 tggtgggagc tgcggcctgc ctcggtggag ttaccaggat gacggtgtca ttggtggtca 1681 tcatgtttga attaaccggg ggtctggagt acatcgtgcc cctgatggcg gcggctgtga 1741 ccagcaagtg ggtagctgat gcatttggga aagaaggcat ctacgaggcc cacatccact 1801 taaatgggta ccctttcctt gacgtgaagg acgagtttac tcaccgcaca ctggccaccg 1861 acgtcatgcg gccccggcgg ggagagccgc cactgtcggt gctcacccag gacagcatga 1921 ctgtcgagga cgtggagacg ctcatcaagg agaccgacta caacggcttc cccgtggtgg 1981 tctccagaga ctccgagcgc cTCATTGGAT TTGCCCAGAG GAGGGAACTG ATTCTCGCAA 2041 TAAAGAACGC CAGACAGAGG CAGGAGGGCA TTGTGAGCAA TTCCATCATG TACTTCACGG 2101 AGGAACCCCC CGAGCTGCCG GCCAACAGCC CACATCCCCT GAAGCTGCGG CGCATCCTGA 2161 ACCTCAGCCC GTTTACAGTG ACAGACCACA CTCCGATGGA AACGGTGGTG GATATCTTCC 2221 GGAAACTGGG GCTTCGGCAG TGCCTGGTGA CGCGGAGCGG GAGACTTCTT GGCATCATCA 2281 CAAAAAAGGA TGTTCTGAGA CATATGGCCC AGATGGCAAA CCAGGACCCC GAATCCATCA 2341 TGTTTAATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2401 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2461 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGAATT AAGGAGCTCT CTAGTAACAC 2521 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt