Construct: ORF TRCN0000466262
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015695.1_s317c1
- Derived from:
- ccsbBroadEn_09733
- DNA Barcode:
- AGGCACCTAGTCAATTGGCTGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC60 (160777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466262
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160777 | CCDC60 | coiled-coil domain containi... | NM_178499.5 | 99.8% | 99.8% | 655A>G;717C>A |
| 2 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018911.2 | 93.3% | 93.2% | 449_450ins108;547A>G;609C>A |
| 3 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018912.2 | 91.8% | 91.8% | 655A>G;717C>A;1229_1230ins132 |
| 4 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018913.2 | 76% | 75.8% | 655A>G;717C>A;967_968ins393 |
| 5 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018914.1 | 67.6% | 66.7% | (many diffs) |
| 6 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018915.1 | 59.2% | 59.2% | 0_1ins672;45C>A |
| 7 | human | 160777 | CCDC60 | coiled-coil domain containi... | XM_017018916.1 | 59.2% | 59.2% | 0_1ins672;45C>A |
| 8 | human | 160777 | CCDC60 | coiled-coil domain containi... | XR_944504.2 | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caaggttcca gccaccaaga agcttcagag ttcccccaac tcgggggctg 121 tccggccctt ttatgcctcg gagaacctaa ggcaggtccc agacaagcca atgaagagca 181 tcaagtatat ggacaaggaa ataataaacc tcaaaaagga ccttatacga agccgctttt 241 tgatccagtc tgtgaagata ggccgtggat attttgctat tctgagggaa gagactgcaa 301 agaaaaagaa gcaacaacaa cttcagaaac tgaaagagga ggaaagaaat aaattccagc 361 cagccgaaaa gatctcagaa atccactatg gggacacctt attgagcaca tatgatgatg 421 agaagttgaa gacactggga gctagagtca cacgtcgccc attcactccc atccacagct 481 gcatcatttc tccctcgcta accgaggctc acgtcgagcc cctcttccgc cagctctgtg 541 ctctccactg gcttctggag gccctgacta ttgaccacac ccaccacacc atgaagcctg 601 tgatcacctg ctggaaccca aaggacccgg gtggaagcaa gagcaccatt aaaaaaatca 661 ataaggacaa gtccatggga cagaaatggg agcatttcat cacagcgcca aagaccaagg 721 aattcaaaat tcccacaatg cgagtcacca accgcaaacc aagccggcga ggctccacac 781 taagtctgag tcgggccagt ggggggtcct ctccccagag cagcatgatc tctgtgaacc 841 ctggctcgga tgagccccca agtgtgaaca cccaggtgac cagcagcaag gacattgagg 901 acaatgagtc atcttcaacc aagccagatg aagaacctct gtatatgaat ctgcagaagc 961 tcctggagat ggttcgggaa gatgcccgga ggacagtcac aatagaaaat gggatgcaaa 1021 gaaaagcacc cagcatcttg tcagtgctga aacaaaacaa gagtaattct gcttataagg 1081 aaatgcagac cactctcaaa tcaagtgaga gatccagcag tacaagtgca gaaagccaca 1141 tccaaccagt ccagaagaag tctaaaaacc gcactaattg tgacatcaac atccactaca 1201 agagtggggt gtgtaacacc atgagggcca agttttacag cgtagcccag gaggctggct 1261 tctgcctgca ggacaagatg gaaatccTCA TGAAGCGCCA AGAAGAGAGA GGTATCCAGA 1321 AGTTCCGTGC TTTTGTCCTT GTCTCAAATT TTCAAAAGGA CATAGCAAAA ATGAGACATC 1381 ACATATCTGT AGTAAAAGGA GATGCAGAAG AAATTGCAGA CCACTGGTAC TTTGATCTGT 1441 TGTCCAAACT GCCAGAGGAT CTAAAGAACT TCCGCCCCGC CAAAAAGATC CTGGTGAAAC 1501 TGCAGAAGTT TGGAGAAAAC CTGGACTTGC GGATTCGACC CCATGTCCTC CTGAAGGTGC 1561 TGCAGGATCT GAGGATTTGG GAACTGTGCT CCCCTGACAT CGCTGTGGCT ATTGAGTTTG 1621 TGCGAGAACA CATCATCCAT ATGCCTCAAG AGGATTACAT CAGCTGGCTG CAGAGCCGGA 1681 TCAACATACC CATTGGGCCC TACAGCGCCC TGAGGTACCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 AAGGCACCTA GTCAATTGGC TGAACACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt