Construct: ORF TRCN0000466270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003247.1_s317c1
- Derived from:
- ccsbBroadEn_07349
- DNA Barcode:
- TGATTATCATCGAGACCCGACAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP7 (9053)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9053 | MAP7 | microtubule associated prot... | NM_003980.4 | 99.9% | 99.8% | 1672C>T;1968C>T |
| 2 | human | 9053 | MAP7 | microtubule associated prot... | XM_006715601.4 | 98.8% | 98.8% | 749_772del;1696C>T;1992C>T |
| 3 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198615.1 | 97.1% | 96.5% | (many diffs) |
| 4 | human | 9053 | MAP7 | microtubule associated prot... | XM_011536243.2 | 96.1% | 95.5% | (many diffs) |
| 5 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198608.1 | 96% | 94.1% | (many diffs) |
| 6 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198614.1 | 96% | 94.1% | (many diffs) |
| 7 | human | 9053 | MAP7 | microtubule associated prot... | XM_006715600.3 | 96% | 94.1% | (many diffs) |
| 8 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198609.1 | 95% | 93.2% | (many diffs) |
| 9 | human | 9053 | MAP7 | microtubule associated prot... | XM_006715598.3 | 95% | 93.2% | (many diffs) |
| 10 | human | 9053 | MAP7 | microtubule associated prot... | XM_006715599.3 | 95% | 93.2% | (many diffs) |
| 11 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198616.1 | 94.9% | 94.9% | 524_525ins111;1561C>T;1857C>T |
| 12 | human | 9053 | MAP7 | microtubule associated prot... | XM_011536245.2 | 93.9% | 93.9% | (many diffs) |
| 13 | human | 9053 | MAP7 | microtubule associated prot... | XM_011536246.2 | 91.2% | 90.6% | (many diffs) |
| 14 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198611.1 | 91.2% | 89.3% | (many diffs) |
| 15 | human | 9053 | MAP7 | microtubule associated prot... | XM_017011471.2 | 91.2% | 89.3% | (many diffs) |
| 16 | human | 9053 | MAP7 | microtubule associated prot... | XM_011536244.3 | 90.3% | 88.4% | (many diffs) |
| 17 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198617.1 | 87.3% | 87.3% | 242_243ins282;1390C>T;1686C>T |
| 18 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198618.1 | 80.4% | 80.3% | 0_1ins438;1234C>T;1530C>T |
| 19 | human | 9053 | MAP7 | microtubule associated prot... | NM_001198619.1 | 80.4% | 80.3% | 0_1ins438;1234C>T;1530C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2313
- ORF length:
- 2247
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagctagga gctggcggcg acggccacag gggcggcgac ggcgcagtgc 121 gaagcgaaac agcacccgac agctacaaag tgcaagataa gaaaaatgcc tccagccgcc 181 ctgcctctgc aatttcagga caaaataaca accactcagg aaataaacca gaccctccgc 241 ctgtgttacg tgttgatgac cggcagcggc tggcccggga gcgacgtgag gaacgggaga 301 aacagctagc tgcaagagaa atagtgtggt tagaaagaga agagcgagcc aggcagcact 361 acgagaagca cctggaagag cggaagaaga ggttggagga gcagaggcag aaggaggagc 421 ggaggagggc tgctgtggag gagaagcgga ggcagagact tgaggaggac aaagaacgcc 481 acgaagctgt tgtacggcgc acaatggaaa ggagccagaa gccaaaacag aagcataacc 541 gttggtcgtg gggaggctct ctccatggga gccctagcat ccacagtgca gatccagaca 601 ggcggtcagt ttccaccatg aatctttcga aatatgttga tcccgtcatt agcaagcggc 661 tctcctcttc atctgcaact ttactaaatt ctccagatag agctcgccgc ctgcagctca 721 gcccatggga gagcagcgtt gttaacagac tcctgacgcc cacacattcg ttcctggcca 781 gaagtaaaag cacagctgcc ttgtctggag aagcagcatc ttgcagcccc atcatcatgc 841 cctacaaagc tgcacactct agaaattcga tggatcgacc aaaactcttt gtaacaccac 901 ctgagggctc ttctcgcagg aggatcattc atggcacagc gagctataaa aaagaaagag 961 agagagaaaa tgtactcttc ctcacatctg gcacccgaag ggctgtatct ccatctaatc 1021 ccaaagcaag acaaccagct cgctcccgac tttggcttcc gtccaagtct cttcctcatt 1081 tgcctggcac acccagaccg acatcctcct tgccacccgg ctcagtcaaa gctgctcctg 1141 ctcaggtccg gcccccatcc cccggcaaca tccgccctgt caagagggaa gtcaaagtgg 1201 agcctgagaa gaaagatcct gagaaggaac ctcagaaagt tgccaatgag ccctcactaa 1261 agggcagagc acctttagtg aaggtagaag aagccacagt tgaagagcgg acacctgctg 1321 aaccagaagt tggccctgct gctccagcca tggccccagc tccagcctcg gccccagctc 1381 cagcctcggc cccagctcca gccccggtcc ccaccccagc catggtctca gccccgtcat 1441 ccactgtgaa tgccagtgct tctgttaaga cttctgcagg caccaccgac ccagaggagg 1501 ccacaaggct tctagctgag aagaggcggc tggcccgaga gcagagagaa aaggaagaaa 1561 gggagaggag ggagcaggaa gagcttgaaa gacaaaagag agaggaattg gctcaacgtg 1621 tggctgaaga gaggacgact cgccgtgagg aggagtcgcg caggctggaa gccgagcagg 1681 cccgggagaa ggaggagcag ctgcagcggc aggcggagga gcgggcgctg cgcgagtggg 1741 aggaggcaga gcgcgcccag aggcagaaag aagaagaagc tcgcgttcgt gaagaagcag 1801 agagggtccg gcaggaacga gagaagcatt tccagagaga agagcaagag cgcctggaga 1861 gaaagaagcg acttgaggag attatgaaaa gaaccaggag aacagaagct acagataaga 1921 aaaccagtga tcagagaaac ggtgatatag ccaagggagc tctcactgga ggaacagagg 1981 tgtctgcact tccatgtaca acaaacgcTC CGGGAAATGG AAAGCCAGTT GGTAGCCCAC 2041 ATGTGGTTAC CTCACACCAG TCAAAAGTGA CAGTGGAGAG CACTCCCGAT TTGGAAAAAC 2101 AACCAAATGA AAATGGTGTA TCTGTTCAGA ATGAAAATTT TGAAGAAATT ATAAACTTAC 2161 CCATTGGATC TAAACCATCC AGATTAGATG TCACCAACAG TGAGAGCCCA GAAATTCCTT 2221 TGAATCCAAT TTTGGCCTTT GATGATGAAG GGACACTTGG GCCCCTGCCT CAGGTAGATG 2281 GTGTTCAGAC ACAGCAGACT GCAGAAGTTA TATGCCCAAC TTTCTTGTAC AAAGTGGTTG 2341 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2401 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 2461 ATTATCATCG AGACCCGACA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2521 ttgtgaaaga tt