Construct: ORF TRCN0000466278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013613.1_s317c1
- Derived from:
- ccsbBroadEn_01384
- DNA Barcode:
- TCGTCGCATGACCCCCTGATAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCN1 (5954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466278
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5954 | RCN1 | reticulocalbin 1 | NM_002901.3 | 100% | 100% | |
| 2 | mouse | 19672 | Rcn1 | reticulocalbin 1 | NM_009037.2 | 87.8% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcgcggtggc cgcggccgcc gcctggggtt agccctgggg ctgctgctgg 121 cgctggtgct ggcgccgcgg gttctgcggg ccaagcccac ggtgcgcaaa gagcgcgtgg 181 tgcggcccga ctcggagctg ggcgagcggc cccctgagga caaccagagc ttccagtacg 241 accacgaggc cttcctgggc aaggaggact ccaagacctt cgaccagctc accccggacg 301 agagcaagga gaggctaggg aagattgttg atcgaatcga caatgatggg gatggctttg 361 tcactactga ggagctgaaa acctggatca aacgggtgca gaaaagatac atctttgata 421 atgtcgccaa agtctggaag gattatgata gggacaagga tgataaaatt tcctgggaag 481 aatacaaaca agccacctat ggttactacc taggaaaccc cgcagagttt catgattctt 541 cagatcatca cacctttaaa aagatgctgc cacgtgatga gagaagattc aaagctgcag 601 acctcaatgg tgacctgaca gctactcggg aggagttcac tgcctttctg catcctgaag 661 agtttgaaca tatgaaggaa attgtggttt tggaaaccct ggaggacatc gacaagaacg 721 gggatgggtt tgtggatcag gatgagtata ttgcggatat gttttcccat gaggagaatg 781 gcccTGAGCC AGACTGGGTT TTATCAGAAC GGGAGCAGTT TAACGAATTC CGGGATCTGA 841 ACAAGGACGG GAAGTTAGAC AAAGATGAGA TTCGCCACTG GATCCTCCCT CAAGATTATG 901 ATCATGCACA GGCTGAGGCC AGGCATCTGG TATATGAATC AGACAAAAAC AAGGATGAGA 961 AGCTAACTAA AGAGGAAATA TTGGAGAACT GGAACATGTT TGTCGGAAGC CAAGCTACCA 1021 ATTACGGGGA AGATCTCACA AAAAATCATG ATGAGCTTTG CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGATCGTCG CATGACCCCC TGATAATTAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt