Construct: ORF TRCN0000466283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013758.1_s317c1
- Derived from:
- ccsbBroadEn_08389
- DNA Barcode:
- ATCAGTCGGTATACTGACAGGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX29 (54505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466283
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54505 | DHX29 | DExH-box helicase 29 | NM_019030.4 | 99.9% | 99.9% | 732A>G;1889C>A;2271A>G |
| 2 | human | 54505 | DHX29 | DExH-box helicase 29 | NM_001345964.2 | 96.2% | 96.2% | 732A>G;1811_1812ins153;2118A>G |
| 3 | human | 54505 | DHX29 | DExH-box helicase 29 | XR_001742128.2 | 93.8% | (many diffs) | |
| 4 | human | 54505 | DHX29 | DExH-box helicase 29 | NR_144324.2 | 87% | (many diffs) | |
| 5 | human | 54505 | DHX29 | DExH-box helicase 29 | NR_144323.2 | 86.5% | (many diffs) | |
| 6 | human | 54505 | DHX29 | DExH-box helicase 29 | NR_144325.2 | 83.8% | (many diffs) | |
| 7 | human | 54505 | DHX29 | DExH-box helicase 29 | NM_001345965.2 | 53.2% | 52.3% | (many diffs) |
| 8 | mouse | 218629 | Dhx29 | DEAH (Asp-Glu-Ala-His) box ... | NM_172594.2 | 84.7% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4173
- ORF length:
- 4107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggcaagaac aagaaacaca aggctccagc ggccgcggtg gtccgggccg 121 ccgtgtctgc ttccagagcc aaatctgccg aggctggaat tgccggggag gcccaaagca 181 agaagccagt gtccaggccg gccaccgctg ccgctgccgc tgccggctcc agggagcccc 241 gtgtcaagca aggtccaaaa atttatagtt ttaattctac aaatgattct agtggtcctg 301 caaatctgga taaatctatt ttgaaagtgg taattaataa caaactagag caaagaatta 361 ttggagtgat caatgagcat aaaaagcaaa ataatgacaa aggaatgatt tctggaagac 421 ttactgccaa aaaattgcag gatttataca tggctttaca agcattttca tttaagacaa 481 aggacattga agatgccatg accaatacac tcttatatgg aggtgacctt cattctgcct 541 tggattggct ctgtttaaac ctttcagatg atgcacttcc tgaaggattc agtcaggaat 601 ttgaagagca gcaacctaaa agtaggccta aatttcagtc tcctcaaata caagccacta 661 tttcacctcc attgcaacct aaaacaaaaa catatgaaga ggaccctaag agtaagccaa 721 aaaaggaaga aaaaaatatg gaagtaaata tgaaagagtg gattttacga tatgctgaac 781 aacaaaatga agaagagaag aatgagaatt ctaaaagttt agaagaggag gaaaaatttg 841 accctaatga aaggtactta catcttgcag caaaactgct ggatgcaaaa gaacaagcag 901 ctacctttaa actagaaaaa aacaagcaag gccaaaaaga ggctcaggaa aaaataagga 961 aatttcaaag agaaatggaa actttagaag accatccagt atttaaccca gccatgaaga 1021 tttcacatca acaaaatgaa aggaaaaagc ctcctgtagc cacagaagga gaaagtgcat 1081 tgaattttaa tttatttgaa aaatctgcag ctgctactga agaagagaaa gataaaaaga 1141 aagaacctca tgatgtaaga aattttgact atactgctcg aagttggact ggaaaatctc 1201 ccaaacaatt tctgattgat tgggtcagga agaatcttcc caagagtcca aatccttcct 1261 ttgaaaaagt tccagtaggt agatactgga aatgtagggt tagggtaatc aagtctgaag 1321 atgatgtcct ggtagtatgc cctacaatct taacagaaga tggcatgcaa gctcagcacc 1381 tgggagctac tttagccctt taccgtttag ttaaagggca gtcagttcat cagttacttc 1441 ctcccactta ccgagatgtt tggctggagt ggagtgatgc agaaaagaaa agggaagaat 1501 taaataaaat ggaaaccaat aaaccacgtg atctttttat tgccaaactt ctgaataaac 1561 tgaaacagca gcaacagcag caacaacagc attctgaaaa taagagagaa aactctgaag 1621 atcccgagga atcttgggaa aatttagttt cggatgagga tttttctgca ctgtccttgg 1681 aatcagcaaa tgtggaagat ttggaacctg ttagaaacct ctttagaaag ttgcaaagca 1741 cacctaagta tcagaaactt ctaaaggaaa gacaacagct acctgtattt aaacatcggg 1801 actcaattgt tgaaactctt aaaaggcatc gggtagtggt tgtggcaggt gaaacaggga 1861 gtggtaaaag tactcaggta ccacattttc tattggaaga tttgcttcta aatgagtggg 1921 aagcaagtaa atgtaacatt gtctgtaccc aacaccgaag aatctcagca gttagtttag 1981 ccaacagagt atgtgatgaa ttgggctgtg aaaatggacc tggaggaagg aattccttgt 2041 gtggatatca gatccggatg gaatctcgag cttgtgaatc taccaggtta ctctattgta 2101 caacaggggt tttgctaagg aaacttcaag aagatggtct tctaagtaat gtgtctcatg 2161 ttattgtaga tgaggttcat gaaagaagtg tccagtcaga cttcctacta attatcttga 2221 aggaaatttt acagaaacgt tctgatctac acttgattct aatgagtgcc actgtggaca 2281 gcgaaaaatt ttctacatat ttcacacact gccccattct cagaatttca ggaaggagtt 2341 atcctgttga ggtttttcat cttgaagata taatagaaga aacaggcttt gtactggaaa 2401 aagactcaga atattgtcag aaatttctgg aagaggaaga agaagtaacc attaatgtta 2461 caagcaaagc agggggaata aaaaaatatc aggaatacat cccagttcag actggagcac 2521 atgctgattt aaatccattt taccaaaagt acagcagccg cactcagcat gctattctat 2581 acatgaatcc tcataaaatc aacctggatc tcattttgga acttcttgca tacttagata 2641 aaagtcccca attcagaaat attgaaggag cagtattgat ctttttacca ggacttgctc 2701 atattcagca gttgtatgat cttctatcaa atgatagaag attttattct gaacgatata 2761 aagtgatagc tctgcattct attctttcaa cccaagatca agctgcagca ttcacacttc 2821 cccctccagg agtcaggaag attgttttag caaccaatat tgcagagacg ggtatcacta 2881 ttcctgatgt tgtatttgta attgatactg gaagaacaaa agaaaataag taccatgaaa 2941 gcagtcagat gagttctttg gtggagacgt ttgtcagtaa agccagtgct ttgcagcgcc 3001 agggaagagc tgggcgggtc agagatggct tctgtttccg aatgtacaca agagaaagat 3061 ttgaaggctt tatggattat tctgttcctg aaatcttacg tgtacctttg gaggaattat 3121 gccttcatat tatgaaatgt aatcttggtt ctcctgaaga tttcctctcc aaagccttag 3181 atcctcctca gctccaagtg atcagcaatg caatgaattt gctccgaaaa attggagctt 3241 gtgaattaaa tgagcctaaa ctgactccgt tgggccaaca ccttgcagct ttacctgtga 3301 atgtcaagat tggcaagatg cttatttttg gtgccatatt tggctgcctt gacccagtgg 3361 caacactagc tgcagttatg acagagaagt ctccttttac cacaccaatt ggtcgaaaag 3421 atgaagcaga tcttgcaaaa tcagctttgg ccatggcgga ttcagaccac ctgacgatct 3481 acaatgcata tctaggatgg aagaaagcac gacaagaagg aggttatcgt tctgaaatca 3541 catactgccg gaggaacttt cttaatagaa catcactgtt aaccctagag gatgtaaagc 3601 aggagttaat aaagttggtt aaggcagcag gattttcatc ttccacaact tctaccagct 3661 gggaaggaaa cagagcctca cagaccctct cattccaaga aattgccctt cttaaagctg 3721 tactggtggc tggactgtat gacaatgtgg ggaagataat ctatacaaag tcagtggatg 3781 ttacagaaaa attggcttgc attgtggaga cggccCAAGG CAAAGCACAA GTACACCCAT 3841 CCTCAGTAAA TCGAGATTTG CAAACTCATG GATGGCTCTT ATACCAGGAG AAGATAAGGT 3901 ATGCCAGAGT GTATTTGAGA GAAACTACCC TAATAACCCC TTTTCCAGTT TTACTTTTTG 3961 GTGGTGATAT AGAAGTTCAG CACCGAGAAC GTCTTCTTTC TATTGATGGC TGGATCTATT 4021 TTCAGGCCCC TGTAAAGATA GCTGTCATTT TCAAGCAGCT GAGAGTTCTC ATTGATTCAG 4081 TTTTAAGAAA AAAGCTTGAA AATCCAAAGA TGTCCCTTGA AAATGACAAG ATTCTGCAGA 4141 TCATTACGGA ATTGATAAAA ACAGAGAATA ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 4201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 4261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 4321 CAGTCGGTAT ACTGACAGGA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 4381 ttgtgaaaga tt