Construct: ORF TRCN0000466284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013920.1_s317c1
- Derived from:
- ccsbBroadEn_07026
- DNA Barcode:
- TTCCCATGAGACACGCAACATGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT1 (6857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6857 | SYT1 | synaptotagmin 1 | NM_001135805.2 | 99.9% | 100% | 75C>T |
| 2 | human | 6857 | SYT1 | synaptotagmin 1 | NM_001135806.2 | 99.9% | 100% | 75C>T |
| 3 | human | 6857 | SYT1 | synaptotagmin 1 | NM_005639.3 | 99.9% | 100% | 75C>T |
| 4 | human | 6857 | SYT1 | synaptotagmin 1 | XM_011538710.2 | 99.9% | 100% | 75C>T |
| 5 | human | 6857 | SYT1 | synaptotagmin 1 | NM_001291901.2 | 99.2% | 99.2% | 75C>T;349_350insAGGCCCTCA |
| 6 | human | 6857 | SYT1 | synaptotagmin 1 | XM_005269113.3 | 99.2% | 99.2% | 75C>T;349_350insAGGCCCTCA |
| 7 | human | 6857 | SYT1 | synaptotagmin 1 | XM_006719576.1 | 99.2% | 99.2% | 75C>T;349_350insAGGCCCTCA |
| 8 | mouse | 20979 | Syt1 | synaptotagmin I | NM_001252341.1 | 89.9% | 97.6% | (many diffs) |
| 9 | mouse | 20979 | Syt1 | synaptotagmin I | NM_009306.3 | 89.9% | 97.6% | (many diffs) |
| 10 | mouse | 20979 | Syt1 | synaptotagmin I | XM_006513446.1 | 89.9% | 97.6% | (many diffs) |
| 11 | mouse | 20979 | Syt1 | synaptotagmin I | XM_006513447.1 | 89.9% | 97.6% | (many diffs) |
| 12 | mouse | 20979 | Syt1 | synaptotagmin I | XM_006513448.1 | 89.9% | 97.6% | (many diffs) |
| 13 | mouse | 20979 | Syt1 | synaptotagmin I | XM_017313866.1 | 89.9% | 97.6% | (many diffs) |
| 14 | mouse | 20979 | Syt1 | synaptotagmin I | NM_001252342.1 | 89.3% | 96.9% | (many diffs) |
| 15 | mouse | 20979 | Syt1 | synaptotagmin I | XM_006513449.1 | 89.3% | 96.9% | (many diffs) |
| 16 | mouse | 20979 | Syt1 | synaptotagmin I | XM_017313867.1 | 89.3% | 96.9% | (many diffs) |
| 17 | mouse | 20979 | Syt1 | synaptotagmin I | XM_017313868.1 | 89.3% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gagcgagagt caccatgagg ccctggcagc cccgcctgtc accactgtcg 121 cgactgttct gccaagcaat gccacagagc cagccagtcc tggagaagga aaggaagatg 181 cattttctaa gctgaaggag aagtttatga atgagttgca taaaattcca ttgccaccgt 241 gggccttaat tgcaatagcc atagtcgcag tccttttagt cctgacctgc tgcttttgta 301 tctgtaagaa atgtttgttc aaaaagaaaa acaagaagaa gggaaaggaa aaaggaggga 361 agaatgccat taacatgaaa gatgtaaaag acttagggaa gacgatgaaa gatcaggccc 421 tcaaggatga tgatgctgaa actggattga cagatggaga agaaaaagaa gaacccaaag 481 aagaggagaa actgggaaaa cttcagtatt cactggatta tgatttccaa aataaccagc 541 tgctggtagg gatcattcag gctgccgaac tgcccgcctt ggacatgggg ggcacatctg 601 atccttacgt gaaagtgttt ctgctacctg ataagaagaa gaaatttgag acaaaagtcc 661 accgaaaaac ccttaatcct gtcttcaatg agcaatttac tttcaaggta ccatactcgg 721 aattgggtgg caaaacccta gtgatggctg tatatgattt tgatcgtttc tctaagcatg 781 acatcattgg agaatttaaa gtccctatga acacagtgga ttttggccat gtaactgagg 841 aatggcgtga cctgcaaagt gctgagaagg aagagcaaga gaaattgggt gatatctgct 901 tctcccttcg ctacgtacct actgctggta agctgactgt tgtcattctg gaggcaaaga 961 acctgaagaa gatggatgtg ggtggcttat ccgatccTTA TGTGAAGATT CATCTGATGC 1021 AGAATGGTAA GAGGCTGAAG AAGAAAAAGA CAACAATTAA AAAGAACACA CTTAACCCCT 1081 ACTACAATGA GTCATTCAGC TTTGAAGTAC CTTTTGAACA AATCCAGAAA GTGCAGGTGG 1141 TGGTAACTGT TTTGGACTAT GACAAGATTG GCAAGAACGA TGCCATCGGC AAAGTCTTTG 1201 TGGGCTACAA CAGCACCGGC GCGGAGCTGC GACACTGGTC AGACATGCTG GCCAACCCCA 1261 GGCGACCTAT TGCCCAGTGG CACACCCTGC AGGTAGAGGA GGAAGTTGAT GCCATGCTGG 1321 CCGTCAAGAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC CCATGAGACA CGCAACATGT 1501 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t