Construct: ORF TRCN0000466284

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013920.1_s317c1
Derived from:
ccsbBroadEn_07026
DNA Barcode:
TTCCCATGAGACACGCAACATGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SYT1 (6857)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466284
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6857 SYT1 synaptotagmin 1 NM_001135805.2 99.9% 100% 75C>T
2 human 6857 SYT1 synaptotagmin 1 NM_001135806.2 99.9% 100% 75C>T
3 human 6857 SYT1 synaptotagmin 1 NM_005639.3 99.9% 100% 75C>T
4 human 6857 SYT1 synaptotagmin 1 XM_011538710.2 99.9% 100% 75C>T
5 human 6857 SYT1 synaptotagmin 1 NM_001291901.2 99.2% 99.2% 75C>T;349_350insAGGCCCTCA
6 human 6857 SYT1 synaptotagmin 1 XM_005269113.3 99.2% 99.2% 75C>T;349_350insAGGCCCTCA
7 human 6857 SYT1 synaptotagmin 1 XM_006719576.1 99.2% 99.2% 75C>T;349_350insAGGCCCTCA
8 mouse 20979 Syt1 synaptotagmin I NM_001252341.1 89.9% 97.6% (many diffs)
9 mouse 20979 Syt1 synaptotagmin I NM_009306.3 89.9% 97.6% (many diffs)
10 mouse 20979 Syt1 synaptotagmin I XM_006513446.1 89.9% 97.6% (many diffs)
11 mouse 20979 Syt1 synaptotagmin I XM_006513447.1 89.9% 97.6% (many diffs)
12 mouse 20979 Syt1 synaptotagmin I XM_006513448.1 89.9% 97.6% (many diffs)
13 mouse 20979 Syt1 synaptotagmin I XM_017313866.1 89.9% 97.6% (many diffs)
14 mouse 20979 Syt1 synaptotagmin I NM_001252342.1 89.3% 96.9% (many diffs)
15 mouse 20979 Syt1 synaptotagmin I XM_006513449.1 89.3% 96.9% (many diffs)
16 mouse 20979 Syt1 synaptotagmin I XM_017313867.1 89.3% 96.9% (many diffs)
17 mouse 20979 Syt1 synaptotagmin I XM_017313868.1 89.3% 96.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1332
ORF length:
1266
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gagcgagagt caccatgagg ccctggcagc cccgcctgtc accactgtcg
121cgactgttct gccaagcaat gccacagagc cagccagtcc tggagaagga aaggaagatg
181cattttctaa gctgaaggag aagtttatga atgagttgca taaaattcca ttgccaccgt
241gggccttaat tgcaatagcc atagtcgcag tccttttagt cctgacctgc tgcttttgta
301tctgtaagaa atgtttgttc aaaaagaaaa acaagaagaa gggaaaggaa aaaggaggga
361agaatgccat taacatgaaa gatgtaaaag acttagggaa gacgatgaaa gatcaggccc
421tcaaggatga tgatgctgaa actggattga cagatggaga agaaaaagaa gaacccaaag
481aagaggagaa actgggaaaa cttcagtatt cactggatta tgatttccaa aataaccagc
541tgctggtagg gatcattcag gctgccgaac tgcccgcctt ggacatgggg ggcacatctg
601atccttacgt gaaagtgttt ctgctacctg ataagaagaa gaaatttgag acaaaagtcc
661accgaaaaac ccttaatcct gtcttcaatg agcaatttac tttcaaggta ccatactcgg
721aattgggtgg caaaacccta gtgatggctg tatatgattt tgatcgtttc tctaagcatg
781acatcattgg agaatttaaa gtccctatga acacagtgga ttttggccat gtaactgagg
841aatggcgtga cctgcaaagt gctgagaagg aagagcaaga gaaattgggt gatatctgct
901tctcccttcg ctacgtacct actgctggta agctgactgt tgtcattctg gaggcaaaga
961acctgaagaa gatggatgtg ggtggcttat ccgatccTTA TGTGAAGATT CATCTGATGC
1021AGAATGGTAA GAGGCTGAAG AAGAAAAAGA CAACAATTAA AAAGAACACA CTTAACCCCT
1081ACTACAATGA GTCATTCAGC TTTGAAGTAC CTTTTGAACA AATCCAGAAA GTGCAGGTGG
1141TGGTAACTGT TTTGGACTAT GACAAGATTG GCAAGAACGA TGCCATCGGC AAAGTCTTTG
1201TGGGCTACAA CAGCACCGGC GCGGAGCTGC GACACTGGTC AGACATGCTG GCCAACCCCA
1261GGCGACCTAT TGCCCAGTGG CACACCCTGC AGGTAGAGGA GGAAGTTGAT GCCATGCTGG
1321CCGTCAAGAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1381ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1441CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC CCATGAGACA CGCAACATGT
1501AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)