Transcript: Mouse XM_017313867.1

PREDICTED: Mus musculus synaptotagmin I (Syt1), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Syt1 (20979)
Length:
4814
CDS:
668..1924

Additional Resources:

NCBI RefSeq record:
XM_017313867.1
NBCI Gene record:
Syt1 (20979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017313867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000093254 CCTCAGTAATATGGGTCCTTT pLKO.1 1987 3UTR 100% 4.950 6.930 N Syt1 n/a
2 TRCN0000053857 GCAAAGTCTTTGTGGGCTACA pLKO.1 1779 CDS 100% 4.050 5.670 N SYT1 n/a
3 TRCN0000093255 GCCTTAATTGCCATAGCCATA pLKO.1 842 CDS 100% 4.050 5.670 N Syt1 n/a
4 TRCN0000093256 GCTTCAATATTCACTGGACTA pLKO.1 1090 CDS 100% 4.050 3.240 N Syt1 n/a
5 TRCN0000093257 CAGTCTTCAATGAACAGTTTA pLKO.1 1269 CDS 100% 13.200 9.240 N Syt1 n/a
6 TRCN0000379704 GGGAGGGAAGAACGCCATTAA pLKO_005 952 CDS 100% 13.200 9.240 N Syt1 n/a
7 TRCN0000381596 CATCTGATCCATACGTCAAAG pLKO_005 1185 CDS 100% 10.800 7.560 N Syt1 n/a
8 TRCN0000380868 CCATGCATTCCTGATACAATC pLKO_005 2018 3UTR 100% 10.800 7.560 N Syt1 n/a
9 TRCN0000380921 TGAAGTTCCGTTCGAGCAAAT pLKO_005 1693 CDS 100% 10.800 7.560 N Syt1 n/a
10 TRCN0000093258 GAGCAAATCCAGAAAGTGCAA pLKO.1 1706 CDS 100% 2.640 1.848 N Syt1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017313867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07026 pDONR223 100% 89.3% 96.9% None (many diffs) n/a
2 ccsbBroad304_07026 pLX_304 0% 89.3% 96.9% V5 (many diffs) n/a
3 TRCN0000466284 TTCCCATGAGACACGCAACATGTA pLX_317 26.8% 89.3% 96.9% V5 (many diffs) n/a
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